HitPredict

Interactor 1: PAK4 (O96013)
Serine/threonine-protein kinase PAK 4 (EC 2.7.11.1) (p21-activated kinase 4) (PAK-4)
Gene: PAK4

Interactor 2: 1433T (P27348)
14-3-3 protein theta (14-3-3 protein T-cell) (14-3-3 protein tau) (Protein HS1)
Gene: YWHAQ

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.94	0.60	0.752

Property	Details
Experiments	32707033, 17353931, 33961781, 35271311, 36931259, 15161933, 28514442, unassigned3799
Identification Methods	affinity chromatography technology, proximity labelling technology, pull down, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005925 - focal adhesion GO:0007165 - signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 591 Hs			1 245
+	Hs
		354631 PAK4_HUMAN (1-591): 0: 598: 100.00		1433E_HUMAN (2-243): 1.32e-144: 406: 63.53
+	Hs
		354656 PAK4_HUMAN (1-591): 0: 598: 100.00		1433Z_HUMAN (1-245): 1.76e-141: 397: 79.59
+	Hs
		354559 PAK4_HUMAN (1-591): 0: 598: 100.00		1433B_HUMAN (1-242): 5.04e-139: 391: 81.82
+	Hs
		354560 PAK4_HUMAN (1-591): 0: 598: 100.00		1433S_HUMAN (1-236): 9.9e-108: 312: 71.85
+	Hs
		972035 PAK6_HUMAN (1-591): 5.36e-170: 500: 45.72		1433E_HUMAN (2-243): 1.32e-144: 406: 63.53
+	Hs
		354676 PAK4_HUMAN (1-591): 0: 598: 100.00		1433F_HUMAN (2-242): 7.19e-106: 307: 71.43
+	Hs
		354625 PAK4_HUMAN (1-591): 0: 598: 100.00		1433G_HUMAN (1-242): 5.51e-105: 305: 70.33
+	Hs
		691463 PAK6_HUMAN (1-591): 5.36e-170: 500: 45.72		1433T_HUMAN (1-245): 9.51e-142: 398: 100.00
+	Hs
		990774 PAK6_HUMAN (1-591): 5.36e-170: 500: 45.72		1433Z_HUMAN (1-245): 1.76e-141: 397: 79.59
+	Hs
		730275 PAK6_HUMAN (1-591): 5.36e-170: 500: 45.72		1433B_HUMAN (1-242): 5.04e-139: 391: 81.82
+	Hs
		730908 PAK6_HUMAN (1-591): 5.36e-170: 500: 45.72		1433S_HUMAN (1-236): 9.9e-108: 312: 71.85
+	Hs
		1049819 PAK6_HUMAN (1-591): 5.36e-170: 500: 45.72		1433F_HUMAN (2-242): 7.19e-106: 307: 71.43
+	Hs
		966912 PAK6_HUMAN (1-591): 5.36e-170: 500: 45.72		1433G_HUMAN (1-242): 5.51e-105: 305: 70.33
+	Hs
		971584 MYLK3_HUMAN (95-580): 2.74e-128: 397: 23.48		1433E_HUMAN (2-243): 1.32e-144: 406: 63.53
+	At
		1030844 CDPK1_ARATH (182-591): 2.67e-131: 398: 23.88		14332_ARATH (3-243): 8.69e-144: 404: 61.22
+	At
		1030913 CIPKC_ARATH (302-577): 3.72e-132: 397: 25.18		14332_ARATH (3-243): 8.69e-144: 404: 61.22
+	At
		840534 CIPKC_ARATH (302-577): 3.72e-132: 397: 25.18		14333_ARATH (3-243): 8.04e-143: 401: 61.63
+	At
		1379653 CIPKC_ARATH (302-577): 3.72e-132: 397: 25.18		14337_ARATH (2-243): 2.79e-142: 400: 60.32
+	At
		854983 CDPK1_ARATH (182-591): 2.67e-131: 398: 23.88		14334_ARATH (3-243): 2.71e-141: 398: 61.63
+	Hs
		730628 MYLK3_HUMAN (95-580): 2.74e-128: 397: 23.48		1433S_HUMAN (1-236): 9.9e-108: 312: 71.85