HitPredict

Interactor 1: RPOC (P0A8T7)
DNA-directed RNA polymerase subunit beta' (RNAP subunit beta') (EC 2.7.7.6) (RNA polymerase subunit beta') (Transcriptase subunit beta')
Gene: RPOC

Interactor 2: RPOB (P0A8V2)
DNA-directed RNA polymerase subunit beta (RNAP subunit beta) (EC 2.7.7.6) (RNA polymerase subunit beta) (Transcriptase subunit beta)
Gene: RPOB

Species: Escherichia coli K-12

Source: intact

Category: Small-scale

Confidence: High

Method score Annotation score Interaction score
0.85 1.00 0.926

The method score is based on:

Property Details
Experiments 16606699, 19903881, 24402227, 15690043, 17960736
Identification Methods affinity chromatography technology, pull down, tandem affinity purification, cross-linking study
Interaction Type physical association, association

The annotation score is based on:

Property Details
Interacting Pfam domains RNA_pol_Rpb1_1~RNA_pol_Rpb2_1, RNA_pol_Rpb1_1~RNA_pol_Rpb2_6, RNA_pol_Rpb1_1~RNA_pol_Rpb2_7, RNA_pol_Rpb1_2~RNA_pol_Rpb2_6, RNA_pol_Rpb1_2~RNA_pol_Rpb2_7, RNA_pol_Rpb1_3~RNA_pol_Rpb2_1, RNA_pol_Rpb1_3~RNA_pol_Rpb2_6, RNA_pol_Rpb1_4~RNA_pol_Rpb2_1, RNA_pol_Rpb1_4~RNA_pol_Rpb2_3, RNA_pol_Rpb1_4~RNA_pol_Rpb2_45, RNA_pol_Rpb1_4~RNA_pol_Rpb2_6, RNA_pol_Rpb1_5~RNA_pol_Rpb2_1, RNA_pol_Rpb1_5~RNA_pol_Rpb2_3, RNA_pol_Rpb1_5~RNA_pol_Rpb2_45, RNA_pol_Rpb1_5~RNA_pol_Rpb2_6, RNA_pol_Rpb1_5~RNA_pol_Rpb2_7
Common GO terms GO:0000345 - cytosolic DNA-directed RNA polymerase complex
GO:0003677 - DNA binding
GO:0003899 - DNA-directed 5'-3' RNA polymerase activity
GO:0005737 - cytoplasm
GO:0005829 - cytosol
and 15 others

more...
Homologous interactions 20

Homologous interactions (Top 20)

Color coding for hit scores

1 1407
Ec

1 1342
+ Nv
+ Hs
+ Bt
+ Hs
+ Dm
+ Sp
+ Hs
+ Hs
+ Pf
+ At
+ At
+ Hs
+ Hs
+ Sc
+ Hs
+ Hv
+ Sc
+ Hv
+ Hs
+ Hs

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Last updated: 15 Jul 2024