HitPredict

Interactor 1: ACTN1 (P12814)
Alpha-actinin-1 (Alpha-actinin cytoskeletal isoform) (F-actin cross-linking protein) (Non-muscle alpha-actinin-1)
Gene: ACTN1

Interactor 2: DAPK1 (P53355)
Death-associated protein kinase 1 (DAP kinase 1) (EC 2.7.11.1)
Gene: DAPK1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	1.00	0.691

Property	Details
Experiments	32707033
Identification Methods	affinity chromatography technology, pull down
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	EF-hand_6~Pkinase
Common GO terms	GO:0005737 - cytoplasm GO:0005886 - plasma membrane GO:0042981 - regulation of apoptotic process GO:0098978 - glutamatergic synapse
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 892 Hs			1 1430
+	Hs
		211541 ACTN4_HUMAN (11-892): 0: 642: 86.74		DAPK1_HUMAN (1-1430): 0: 1999: 100.00
+	Hs
		164586 SPTN2_HUMAN (225-763): 1.02e-15: 84.3: 14.42		DAPK1_HUMAN (1-1430): 0: 1999: 100.00
+	Hs
		862027 SPTB2_HUMAN (196-761): 3.97e-14: 79.3: 12.17		DAPK1_HUMAN (1-1430): 0: 1999: 100.00
+	Rn
		506781 ACTN1_RAT (1-892): 0: 698: 99.33		KCC2A_RAT (1-383): 5.07e-120: 385: 29.17
+	Hs
		521641 ACTN1_HUMAN (1-892): 0: 697: 100.00		KCC2A_HUMAN (1-383): 6.29e-120: 385: 29.17
+	Mm
		515254 ACTN2_MOUSE (10-892): 0: 643: 80.63		KCC2A_MOUSE (1-383): 5.07e-120: 385: 29.17
+	Hs
		211695 ACTN4_HUMAN (11-892): 0: 642: 86.74		KCC2A_HUMAN (1-383): 6.29e-120: 385: 29.17
+	Rn
		88556 D3ZCV0_RAT (13-892): 0: 638: 80.25		KCC2A_RAT (1-383): 5.07e-120: 385: 29.17
+	Hs
		164604 SPTN2_HUMAN (225-763): 1.02e-15: 84.3: 14.42		ANK2_HUMAN (248-797): 3.54e-109: 385: 19.42
+	Hs
		963292 SPTB2_HUMAN (196-761): 3.97e-14: 79.3: 12.17		ANK2_HUMAN (248-797): 3.54e-109: 385: 19.42
+	Mm
		513797 DMD_MOUSE (399-699): 1.61e-09: 64.3: 17.86		KCC2D_MOUSE (2-383): 1.06e-116: 377: 27.94
+	Mm
		103603 SPTB2_MOUSE (196-761): 1.13e-14: 81.2: 12.88		ANK3_MOUSE (518-936): 2.88e-74: 276: 16.06
+	Mm
		103598 Q3UGX2_MOUSE (247-856): 1.73e-11: 70.4: 12.19		ANK3_MOUSE (518-936): 2.88e-74: 276: 16.06
+	Hs
		556695 SPTB2_HUMAN (196-761): 3.97e-14: 79.3: 12.17		ANK1_HUMAN (503-915): 9.04e-72: 267: 15.66
+	Hs
		506803 SPTB1_HUMAN (235-847): 3.12e-09: 63.1: 10.29		ANK1_HUMAN (503-915): 9.04e-72: 267: 15.66
+	Mm
		57853 SPTB2_MOUSE (196-761): 1.13e-14: 81.2: 12.88		KALRN_MOUSE (2-312): 1.12e-61: 236: 38.91
+	Mm
		57856 Q68FG2_MOUSE (225-763): 5.44e-14: 78.9: 13.87		KALRN_MOUSE (2-312): 1.12e-61: 236: 38.91
+	Hs
		164611 SPTN2_HUMAN (225-763): 1.02e-15: 84.3: 14.42		ANK3_HUMAN (737-962): 6.16e-11: 69.6: 12.16
+	Hs
		963306 SPTB2_HUMAN (196-761): 3.97e-14: 79.3: 12.17		ANK3_HUMAN (737-962): 6.16e-11: 69.6: 12.16
+	Hs
		1021458 SPTN4_HUMAN (173-588): 8.58e-09: 62: 10.82		ANK3_HUMAN (737-962): 6.16e-11: 69.6: 12.16