HitPredict

Interactor 1: TIMP2 (P16035)
Metalloproteinase inhibitor 2 (CSC-21K) (Tissue inhibitor of metalloproteinases 2) (TIMP-2)
Gene: TIMP2

Interactor 2: MMP14 (P50281)
Matrix metalloproteinase-14 (MMP-14) (EC 3.4.24.80) (MMP-X1) (Membrane-type matrix metalloproteinase 1) (MT-MMP 1) (MTMMP1) (Membrane-type-1 matrix metalloproteinase) (MT1-MMP) (MT1MMP)
Gene: MMP14

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.90	1.00	0.950

Property	Details
Experiments	9422789, 8804434, 33961781, 12475252, 19010413, 24112707
Identification Methods	affinity chromatography technology, pull down, surface plasmon resonance, protease assay, anti tag coimmunoprecipitation, anti bait coimmunoprecipitation
Interaction Type	physical association, direct interaction, enzymatic reaction, association

Property	Details
Interacting Pfam domains	Hemopexin~TIMP, Peptidase_M10~TIMP
Common GO terms	GO:0005615 - extracellular space GO:0008270 - zinc ion binding GO:0031012 - extracellular matrix
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 220 Hs			1 582
+	Hs
		554810 TIMP2_HUMAN (1-220): 2.84e-111: 319: 100.00		MMP16_HUMAN (33-582): 0: 759: 55.46
+	Hs
		838537 TIMP4_HUMAN (24-220): 3.3e-107: 309: 49.75		MMP14_HUMAN (1-582): 0: 768: 100.00
+	Hs
		726505 TIMP3_HUMAN (10-220): 1.96e-95: 278: 44.19		MMP14_HUMAN (1-582): 0: 768: 100.00
+	Hs
		344795 TIMP1_HUMAN (10-203): 5.81e-86: 255: 42.29		MMP14_HUMAN (1-582): 0: 768: 100.00
+	Hs
		554809 TIMP2_HUMAN (1-220): 2.84e-111: 319: 100.00		MMP15_HUMAN (27-582): 0: 685: 52.48
+	Hs
		439263 TIMP2_HUMAN (1-220): 2.84e-111: 319: 100.00		MMP3_HUMAN (11-508): 0: 580: 35.77
+	Hs
		439275 TIMP4_HUMAN (24-220): 3.3e-107: 309: 49.75		MMP3_HUMAN (11-508): 0: 580: 35.77
+	Hs
		372726 TIMP2_HUMAN (1-220): 2.84e-111: 319: 100.00		MMP1_HUMAN (16-508): 0: 567: 35.34
+	Hs
		372742 TIMP4_HUMAN (24-220): 3.3e-107: 309: 49.75		MMP1_HUMAN (16-508): 0: 567: 35.34
+	Hs
		439265 TIMP3_HUMAN (10-220): 1.96e-95: 278: 44.19		MMP3_HUMAN (11-508): 0: 580: 35.77
+	Hs
		344781 TIMP1_HUMAN (10-203): 5.81e-86: 255: 42.29		MMP3_HUMAN (11-508): 0: 580: 35.77
+	Mm
		706099 TIMP3_MOUSE (10-220): 1.95e-92: 271: 43.72		MMP13_MOUSE (10-504): 0: 561: 32.81
+	Hs
		344777 TIMP1_HUMAN (10-203): 5.81e-86: 255: 42.29		MMP1_HUMAN (16-508): 0: 567: 35.34
+	Hs
		344784 TIMP1_HUMAN (10-203): 5.81e-86: 255: 42.29		MMP10_HUMAN (11-508): 0: 565: 35.32
+	Hs
		439223 TIMP2_HUMAN (1-220): 2.84e-111: 319: 100.00		MMP2_HUMAN (4-226): 4.4e-79: 264: 34.78
+	Hs
		439254 TIMP4_HUMAN (24-220): 3.3e-107: 309: 49.75		MMP2_HUMAN (4-226): 4.4e-79: 264: 34.78
+	Hs
		540409 TIMP2_HUMAN (1-220): 2.84e-111: 319: 100.00		MMP9_HUMAN (4-224): 6.56e-65: 227: 36.20
+	Hs
		439230 TIMP3_HUMAN (10-220): 1.96e-95: 278: 44.19		MMP2_HUMAN (4-226): 4.4e-79: 264: 34.78
+	Hs
		344780 TIMP1_HUMAN (10-203): 5.81e-86: 255: 42.29		MMP2_HUMAN (4-226): 4.4e-79: 264: 34.78
+	Hs
		344786 TIMP1_HUMAN (10-203): 5.81e-86: 255: 42.29		MMP9_HUMAN (4-224): 6.56e-65: 227: 36.20