HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: ARAP1 (Q96P48)
Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 (Centaurin-delta-2) (Cnt-d2)
Gene: ARAP1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	1.00	0.691

Property	Details
Experiments	33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	PH~SH3_1
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0007015 - actin filament organization GO:0007165 - signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 1450
+	Hs
		209145 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ARAP1_HUMAN (1-1450): 0: 1074: 100.00
+	Hs
		412284 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		ARAP2_HUMAN (290-1448): 0: 782: 39.53
+	Hs
		64651 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		RHG32_HUMAN (647-1331): 2.29e-86: 314: 16.71
+	Hs
		195783 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ASAP2_HUMAN (322-766): 7.55e-83: 296: 19.59
+	Hs
		64627 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		RHG32_HUMAN (647-1331): 2.29e-86: 314: 16.71
+	Hs
		195770 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ASAP2_HUMAN (322-766): 7.55e-83: 296: 19.59
+	Hs
		551038 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		CHIN_HUMAN (845-1139): 1.19e-70: 247: 22.37
+	Hs
		208987 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		CHIN_HUMAN (845-1139): 1.19e-70: 247: 22.37
+	Hs
		557714 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		RHG21_HUMAN (84-499): 2.61e-25: 116: 10.45
+	Hs
		64658 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		RHG32_HUMAN (647-1331): 2.29e-86: 314: 16.71
+	Hs
		64642 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		RHG32_HUMAN (647-1331): 2.29e-86: 314: 16.71
+	Hs
		655450 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		AGAP2_HUMAN (277-760): 7.44e-84: 302: 18.32
+	Mm
		1112566 SRBS2_MOUSE (46-288): 1.38e-24: 107: 18.85		AGAP2_MOUSE (277-760): 5.12e-81: 293: 17.25
+	Hs
		195787 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		ASAP2_HUMAN (322-766): 7.55e-83: 296: 19.59
+	Dm
		993184 GRAP_DROME (1-61): 1.53e-17: 82.3: 31.15		Q0KHR5_DROME (624-1404): 8.84e-87: 314: 16.88
+	Hs
		195776 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		ASAP2_HUMAN (322-766): 7.55e-83: 296: 19.59
+	Hs
		195795 ITSN1_HUMAN (5-286): 1.55e-16: 83.6: 17.07		ASAP2_HUMAN (322-766): 7.55e-83: 296: 19.59
+	Mm
		70554 B2RQ57_MOUSE (197-356): 1.08e-15: 80.9: 33.95		AGAP2_MOUSE (277-760): 5.12e-81: 293: 17.25
+	Mm
		403434 SRC_MOUSE (5-114): 1.55e-15: 79.7: 22.32		AGAP2_MOUSE (277-760): 5.12e-81: 293: 17.25
+	Hs
		1078833 ITSN1_HUMAN (5-286): 1.55e-16: 83.6: 17.07		RHG31_HUMAN (938-1422): 2.55e-74: 275: 17.72