HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: ASAP1 (Q9ULH1)
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1 (130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein) (ADP-ribosylation factor-directed GTPase-activating protein 1) (ARF GTPase-activating protein 1) (Development and differentiation-enhancing factor 1) (DEF-1) (Differentiation-enhancing factor 1) (PIP2-dependent ARF1 GAP)
Gene: ASAP1

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.58	1.00	0.768

Property	Details
Experiments	17474147, 16636290
Identification Methods	peptide array, enzyme linked immunosorbent assay
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	Ank_2~SH3_1, PH~SH3_1
Common GO terms	GO:0005829 - cytosol GO:0045296 - cadherin binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 1129
+	Hs
		209187 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ASAP1_HUMAN (17-1129): 0: 831: 100.00
+	Hs
		789012 TEC_HUMAN (27-377): 4.7e-38: 146: 17.63		ASAP1_HUMAN (17-1129): 0: 831: 100.00
+	Hs
		195783 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ASAP2_HUMAN (21-967): 0: 647: 55.31
+	Hs
		655502 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		ASAP1_HUMAN (17-1129): 0: 831: 100.00
+	Hs
		412404 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		ASAP1_HUMAN (17-1129): 0: 831: 100.00
+	Hs
		442894 HCK_HUMAN (2-174): 4.07e-20: 93.7: 17.24		ASAP1_HUMAN (17-1129): 0: 831: 100.00
+	Hs
		195770 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ASAP2_HUMAN (21-967): 0: 647: 55.31
+	Hs
		557696 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ACAP1_HUMAN (45-690): 2.77e-153: 477: 21.01
+	Hs
		195787 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		ASAP2_HUMAN (21-967): 0: 647: 55.31
+	Hs
		195776 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		ASAP2_HUMAN (21-967): 0: 647: 55.31
+	Hs
		195795 ITSN1_HUMAN (5-286): 1.55e-16: 83.6: 17.07		ASAP2_HUMAN (21-967): 0: 647: 55.31
+	Hs
		557775 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ARAP1_HUMAN (368-620): 3.83e-58: 222: 24.90
+	Hs
		557688 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		GIT2_HUMAN (446-1074): 4.12e-57: 213: 16.98
+	Hs
		209145 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ARAP1_HUMAN (368-620): 3.83e-58: 222: 24.90
+	Hs
		209052 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		GIT2_HUMAN (446-1074): 4.12e-57: 213: 16.98
+	Hs
		655450 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		AGAP2_HUMAN (379-660): 2.63e-91: 320: 28.32
+	Mm
		1112566 SRBS2_MOUSE (46-288): 1.38e-24: 107: 18.85		AGAP2_MOUSE (379-660): 1.64e-87: 309: 29.02
+	Hs
		655498 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		DP13A_HUMAN (45-460): 7.54e-92: 312: 16.24
+	Hs
		129347 P85B_HUMAN (100-376): 1.85e-21: 98.3: 16.61		DP13A_HUMAN (45-460): 7.54e-92: 312: 16.24
+	Hs
		412284 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		ARAP2_HUMAN (162-622): 8.31e-61: 230: 18.97