HitPredict

Interactor 1: UBA1 (P22515)
Ubiquitin-activating enzyme E1 1 (EC 6.2.1.45)
Gene: UBA1

Interactor 2: UBCX (P29340)
Ubiquitin-conjugating enzyme E2-21 kDa (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme PEX4) (Peroxin-4) (Ubiquitin carrier protein) (Ubiquitin-protein ligase)
Gene: PEX4

Species: Saccharomyces cerevisiae S288C

Source: biogrid

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.60	1.00	0.778

Property	Details
Experiments	9341106, 22085930
Identification Methods	enzymatic study
Interaction Type	direct interaction

Property	Details
Interacting Pfam domains	E1_UFD~UQ_con, ThiF~UQ_con
Common GO terms	GO:0005524 - ATP binding GO:0005634 - nucleus GO:0016567 - protein ubiquitination
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1024 Sc			1 183
+	Sc
		549788 UBA1_YEAST (1-1024): 0: 1089: 100.00		UBC4_YEAST (17-178): 9.51e-65: 197: 30.25
+	Sp
		293848 UBA1_SCHPO (1-1024): 0: 1038: 58.29		UBC4_SCHPO (17-178): 3.02e-65: 198: 27.78
+	Sp
		293847 UBA1_SCHPO (1-1024): 0: 1038: 58.29		UBC2_SCHPO (14-177): 5e-57: 177: 25.61
+	Sc
		600919 UBA1_YEAST (1-1024): 0: 1089: 100.00		UBC1_YEAST (21-181): 1.3e-18: 81.3: 32.30
+	Hs
		48104 UBA6_HUMAN (2-1024): 0: 966: 40.81		UB2D1_HUMAN (17-179): 2.37e-67: 204: 27.61
+	Hs
		48107 UBA6_HUMAN (2-1024): 0: 966: 40.81		UB2D2_HUMAN (17-179): 4.23e-67: 203: 27.61
+	Hs
		48106 UBA6_HUMAN (2-1024): 0: 966: 40.81		UB2D3_HUMAN (17-179): 4.75e-66: 200: 27.61
+	Sc
		410211 UBA1_YEAST (1-1024): 0: 1089: 100.00		UBC2_YEAST (61-159): 3.77e-17: 76.6: 39.39
+	Sp
		251976 UBA1_SCHPO (1-1024): 0: 1038: 58.29		UBC1_SCHPO (57-171): 2.96e-17: 77.8: 36.52
+	Hs
		782203 UBA7_HUMAN (5-1024): 0: 909: 36.99		UB2E1_HUMAN (9-178): 4.23e-60: 187: 24.12
+	Hs
		1282360 SAE2_HUMAN (24-413): 8.43e-82: 280: 15.72		UB2E3_HUMAN (6-178): 3.85e-62: 192: 24.86
+	Hs
		919796 UBA3_HUMAN (790-994): 5.83e-41: 159: 14.55		UB2D2_HUMAN (17-179): 4.23e-67: 203: 27.61
+	Hs
		607026 UBA1_HUMAN (393-622): 1.44e-27: 123: 17.75		UB2D1_HUMAN (17-179): 2.37e-67: 204: 27.61
+	Hs
		607050 UBA1_HUMAN (393-622): 1.44e-27: 123: 17.75		UB2D2_HUMAN (17-179): 4.23e-67: 203: 27.61
+	Hs
		607039 UBA1_HUMAN (393-622): 1.44e-27: 123: 17.75		UB2D3_HUMAN (17-179): 4.75e-66: 200: 27.61
+	Hs
		607028 UBA1_HUMAN (393-622): 1.44e-27: 123: 17.75		UB2E1_HUMAN (9-178): 4.23e-60: 187: 24.12
+	Hs
		607023 UBA1_HUMAN (393-622): 1.44e-27: 123: 17.75		UBE2A_HUMAN (14-177): 4.3e-59: 183: 26.22
+	Hs
		607042 UBA1_HUMAN (393-622): 1.44e-27: 123: 17.75		UBE2N_HUMAN (19-168): 3.28e-53: 168: 31.33
+	Hs
		607046 UBA1_HUMAN (393-622): 1.44e-27: 123: 17.75		UB2G1_HUMAN (41-153): 1.69e-16: 74.7: 37.60
+	Hs
		607041 UBA1_HUMAN (393-622): 1.44e-27: 123: 17.75		UBE2K_HUMAN (15-130): 2.06e-15: 72.4: 35.35