HitPredict

Interactor 1: UBA1 (P22515)
Ubiquitin-activating enzyme E1 1 (EC 6.2.1.45)
Gene: UBA1

Interactor 2: UBC6 (P33296)
Ubiquitin-conjugating enzyme E2 6 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme 6) (Ubiquitin carrier protein UBC6) (Ubiquitin-protein ligase UBC6)
Gene: UBC6

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.866

Property	Details
Experiments	11805837, 33015833, 8396728
Identification Methods	anti tag coimmunoprecipitation, affinity chromatography technology, enzymatic study, pull down
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	E1_UFD~UQ_con, ThiF~UQ_con
Common GO terms	GO:0005524 - ATP binding GO:0005634 - nucleus
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1024 Sc			1 250
+	Sc
		549788 UBA1_YEAST (1-1024): 0: 1089: 100.00		UBC4_YEAST (1-151): 1.39e-65: 202: 28.76
+	Sc
		608714 UBA1_YEAST (1-1024): 0: 1089: 100.00		UBC13_YEAST (1-156): 2.87e-57: 181: 25.63
+	Sp
		293848 UBA1_SCHPO (1-1024): 0: 1038: 58.29		UBC4_SCHPO (5-151): 5.39e-67: 205: 28.19
+	Sp
		193850 UBA1_SCHPO (1-1024): 0: 1038: 58.29		UBC6_SCHPO (1-191): 1.4e-58: 187: 51.74
+	Hs
		48104 UBA6_HUMAN (2-1024): 0: 966: 40.81		UB2D1_HUMAN (5-151): 2.16e-67: 206: 26.85
+	Hs
		48107 UBA6_HUMAN (2-1024): 0: 966: 40.81		UB2D2_HUMAN (5-151): 2.74e-67: 206: 26.85
+	Sc
		608719 UBA1_YEAST (1-1024): 0: 1089: 100.00		UBC7_YEAST (3-124): 1.08e-17: 79.3: 34.31
+	Hs
		48106 UBA6_HUMAN (2-1024): 0: 966: 40.81		UB2D3_HUMAN (5-151): 7.9e-66: 202: 26.85
+	Sc
		410211 UBA1_YEAST (1-1024): 0: 1089: 100.00		UBC2_YEAST (6-137): 1.36e-16: 76.6: 32.62
+	Sp
		116506 UBA1_SCHPO (1-1024): 0: 1038: 58.29		UBC7_SCHPO (1-124): 8.18e-18: 79.7: 35.97
+	Sp
		293847 UBA1_SCHPO (1-1024): 0: 1038: 58.29		UBC2_SCHPO (3-115): 1.57e-17: 78.6: 34.19
+	Hs
		782203 UBA7_HUMAN (5-1024): 0: 909: 36.99		UB2E1_HUMAN (4-151): 1.22e-60: 191: 28.00
+	Hs
		48105 UBA6_HUMAN (2-1024): 0: 966: 40.81		UB2G2_HUMAN (6-124): 1.76e-17: 79: 35.82
+	Hs
		1282360 SAE2_HUMAN (24-413): 8.43e-82: 280: 15.72		UB2E3_HUMAN (4-151): 5.89e-62: 194: 29.33
+	Hs
		919796 UBA3_HUMAN (790-994): 5.83e-41: 159: 14.55		UB2D2_HUMAN (5-151): 2.74e-67: 206: 26.85
+	Hs
		607026 UBA1_HUMAN (393-622): 1.44e-27: 123: 17.75		UB2D1_HUMAN (5-151): 2.16e-67: 206: 26.85
+	Sc
		854609 UBA2_YEAST (25-426): 1.8e-67: 240: 16.18		UBC7_YEAST (3-124): 1.08e-17: 79.3: 34.31
+	Hs
		607028 UBA1_HUMAN (393-622): 1.44e-27: 123: 17.75		UB2E1_HUMAN (4-151): 1.22e-60: 191: 28.00
+	Hs
		607038 UBA1_HUMAN (393-622): 1.44e-27: 123: 17.75		UB2G2_HUMAN (6-124): 1.76e-17: 79: 35.82
+	Hs
		607023 UBA1_HUMAN (393-622): 1.44e-27: 123: 17.75		UBE2A_HUMAN (6-128): 1.51e-16: 75.9: 33.86