HitPredict

Interactor 1: MYL9 (P24844)
Myosin regulatory light polypeptide 9 (20 kDa myosin light chain) (LC20) (MLC-2C) (Myosin RLC) (Myosin regulatory light chain 2, smooth muscle isoform) (Myosin regulatory light chain 9) (Myosin regulatory light chain MRLC1)
Gene: MYL9

Interactor 2: MYH9 (P35579)
Myosin-9 (Cellular myosin heavy chain, type A) (Myosin heavy chain 9) (Myosin heavy chain, non-muscle IIa) (Non-muscle myosin heavy chain A) (NMMHC-A) (Non-muscle myosin heavy chain IIa) (NMMHC II-a) (NMMHC-IIA)
Gene: MYH9

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.66	1.00	0.817

Property	Details
Experiments	16169070, 35271311
Identification Methods	two hybrid, affinity chromatography technology, anti tag coimmunoprecipitation, two hybrid pooling approach
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	EF-hand_7~Myosin_head, EF-hand_7~Myosin_N
Common GO terms	GO:0001725 - stress fiber GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0070527 - platelet aggregation
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 172 Hs			1 1960
+	Ce
		104561 CALM_CAEEL (97-171): 7.52e-16: 72.2: 25.33		G5EBY3_CAEEL (19-1920): 0: 1295: 44.84
+	Hs
		159549 ML12B_HUMAN (1-172): 6.88e-67: 203: 93.02		MYH9_HUMAN (1-1923): 0: 1124: 100.00
+	Hs
		580614 ML12A_HUMAN (15-172): 5.65e-65: 198: 94.30		MYH9_HUMAN (1-1923): 0: 1124: 100.00
+	Hs
		648467 CALL3_HUMAN (32-93): 4.82e-11: 59.3: 25.76		MYH9_HUMAN (1-1923): 0: 1124: 100.00
+	Mm
		1143166 Q6ZWQ9_MOUSE (1-172): 7.6e-67: 203: 93.61		MYH10_MOUSE (943-1959): 8.39e-63: 240: 17.33
+	Hs
		159551 ML12B_HUMAN (1-172): 6.88e-67: 203: 93.02		MYH11_HUMAN (723-1800): 1.94e-59: 229: 16.27
+	Hs
		580616 ML12A_HUMAN (15-172): 5.65e-65: 198: 94.30		MYH11_HUMAN (723-1800): 1.94e-59: 229: 16.27
+	Dm
		773034 SQH_DROME (1-172): 4.82e-61: 188: 80.46		MYSN_DROME (938-1959): 1.1e-59: 230: 16.51
+	Hs
		501101 MLRV_HUMAN (9-166): 3.21e-54: 171: 57.23		MYH1_HUMAN (1063-1960): 1.36e-56: 220: 15.08
+	Dm
		404219 MLR_DROME (3-163): 7.57e-30: 110: 40.61		MYSA_DROME (939-1960): 2.45e-65: 248: 17.74
+	Hs
		159595 ML12B_HUMAN (1-172): 6.88e-67: 203: 93.02		MYH14_HUMAN (1316-1960): 4.14e-32: 139: 15.50
+	Ce
		361111 MLR1_CAEEL (24-170): 1.34e-38: 131: 48.65		MYO4_CAEEL (730-1729): 1.73e-53: 209: 16.65
+	Ce
		361112 MLR2_CAEEL (24-170): 1.02e-38: 131: 48.65		MYO4_CAEEL (730-1729): 1.73e-53: 209: 16.65
+	Hs
		580665 ML12A_HUMAN (15-172): 5.65e-65: 198: 94.30		MYH14_HUMAN (1316-1960): 4.14e-32: 139: 15.50
+	Ce
		361122 MLR1_CAEEL (24-170): 1.34e-38: 131: 48.65		MYO1_CAEEL (1258-1960): 1.1e-43: 177: 15.63
+	Ce
		361123 MLR2_CAEEL (24-170): 1.02e-38: 131: 48.65		MYO1_CAEEL (1258-1960): 1.1e-43: 177: 15.63
+	Hs
		159550 ML12B_HUMAN (1-172): 6.88e-67: 203: 93.02		MYH10_HUMAN (1676-1957): 4.42e-10: 67.4: 16.84
+	Hs
		624342 MYL9_HUMAN (1-172): 1.16e-66: 202: 100.00		MYH10_HUMAN (1676-1957): 4.42e-10: 67.4: 16.84
+	Hs
		580615 ML12A_HUMAN (15-172): 5.65e-65: 198: 94.30		MYH10_HUMAN (1676-1957): 4.42e-10: 67.4: 16.84