HitPredict

Interactor 1: BTF3 (P20290)
Transcription factor BTF3 (Nascent polypeptide-associated complex subunit beta) (NAC-beta) (RNA polymerase B transcription factor 3)
Gene: BTF3

Interactor 2: NACA (Q13765)
Nascent polypeptide-associated complex subunit alpha (NAC-alpha) (Alpha-NAC) (allergen Hom s 2)
Gene: NACA

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.96	1.00	0.981

Property	Details
Experiments	33961781, 33644029, 35271311, 35235311, 34349018, 37071682, 38334954, 28524877, 39358380, 31839598, 10982809, 21203952, 30948508, 24981860, 34535262
Identification Methods	affinity chromatography technology, cross-linking study, pull down, x-ray crystallography, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	NAC~NAC
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005854 - nascent polypeptide-associated complex GO:0015031 - protein transport GO:1905551 - negative regulation of protein localization to endoplasmic reticulum
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 206 Hs			1 215
+	Hs
		630035 BTF3_HUMAN (1-206): 3.52e-94: 275: 100.00		NACA2_HUMAN (1-215): 4.75e-93: 272: 90.23
+	Hs
		176756 BTF3_HUMAN (1-206): 3.52e-94: 275: 100.00		NACAD_HUMAN (4-215): 3.41e-72: 239: 61.64
+	Hs
		1395603 BT3L4_HUMAN (50-206): 5.1e-67: 204: 81.01		NACA2_HUMAN (1-215): 4.75e-93: 272: 90.23
+	Hs
		1117128 BT3L4_HUMAN (50-206): 5.1e-67: 204: 81.01		NACA_HUMAN (1-215): 5.16e-88: 260: 100.00
+	Hs
		176771 BT3L4_HUMAN (50-206): 5.1e-67: 204: 81.01		NACAD_HUMAN (4-215): 3.41e-72: 239: 61.64
+	Dm
		1267472 Q7KM15_DROME (50-201): 3.32e-57: 179: 63.40		NACA_DROME (6-215): 4.44e-71: 217: 61.32
+	Ce
		1166308 BTF3_CAEEL (53-204): 4.9e-59: 184: 63.16		NACA_CAEEL (6-215): 1.21e-67: 207: 54.29
+	Dm
		1267480 Q7KM15_DROME (50-201): 3.32e-57: 179: 63.40		Q9VPV7_DROME (6-215): 3.31e-62: 198: 37.50
+	Dm
		1374592 Q9VPR2_DROME (50-205): 6.69e-44: 157: 59.87		NACA_DROME (6-215): 4.44e-71: 217: 61.32
+	Dm
		1483500 Q9VPR2_DROME (50-205): 6.69e-44: 157: 59.87		Q9VPV7_DROME (6-215): 3.31e-62: 198: 37.50
+	Dm
		1374628 Q9VY34_DROME (50-136): 4.58e-27: 105: 35.63		NACA_DROME (6-215): 4.44e-71: 217: 61.32
+	Dm
		75103 B3DNI1_DROME (50-136): 1.25e-26: 104: 36.78		NACA_DROME (6-215): 4.44e-71: 217: 61.32
+	Dm
		1070277 Q0IGS9_DROME (50-136): 2.91e-26: 103: 34.48		NACA_DROME (6-215): 4.44e-71: 217: 61.32
+	Dm
		1374629 Q9VY63_DROME (50-136): 3.26e-26: 103: 36.78		NACA_DROME (6-215): 4.44e-71: 217: 61.32
+	Pf
		91176 Q8ILK2_PLAF7 (47-203): 3.45e-41: 139: 32.93		C6KT55_PLAF7 (63-214): 2.57e-56: 178: 33.77
+	Sc
		806493 NACB1_YEAST (50-199): 3.73e-48: 156: 36.13		NACA_YEAST (62-214): 8.37e-46: 151: 34.12
+	Dm
		1483629 Q9VY34_DROME (50-136): 4.58e-27: 105: 35.63		Q9VPV7_DROME (6-215): 3.31e-62: 198: 37.50
+	Dm
		75105 B3DNI1_DROME (50-136): 1.25e-26: 104: 36.78		Q9VPV7_DROME (6-215): 3.31e-62: 198: 37.50
+	Dm
		1070278 Q0IGS9_DROME (50-136): 2.91e-26: 103: 34.48		Q9VPV7_DROME (6-215): 3.31e-62: 198: 37.50
+	Sc
		806464 NACB2_YEAST (50-172): 2.42e-43: 144: 34.92		NACA_YEAST (62-214): 8.37e-46: 151: 34.12