HitPredict

Interactor 1: NFYB (P25208)
Nuclear transcription factor Y subunit beta (CAAT box DNA-binding protein subunit B) (Nuclear transcription factor Y subunit B) (NF-YB)
Gene: NFYB

Interactor 2: NC2A (Q14919)
Dr1-associated corepressor (Dr1-associated protein 1) (Negative cofactor 2-alpha) (NC2-alpha)
Gene: DRAP1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.69	1.00	0.834

Property	Details
Experiments	25416956, 16189514
Identification Methods	two hybrid, validated two hybrid, two hybrid prey pooling approach, two hybrid array, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	CBFD_NFYB_HMF~CBFD_NFYB_HMF
Common GO terms	GO:0005634 - nucleus GO:0046982 - protein heterodimerization activity GO:0090575 - RNA polymerase II transcription regulator complex
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 207 Hs			1 205
+	Rn
		990944 NFYB_RAT (1-207): 1.5e-92: 270: 99.03		NFYC_RAT (3-101): 9.72e-40: 140: 28.28
+	Hs
		670077 NFYB_HUMAN (1-207): 6.12e-93: 272: 100.00		NFYC_HUMAN (3-101): 1.31e-37: 137: 28.28
+	At
		1285031 NFYB6_ARATH (8-179): 5.45e-66: 204: 40.81		NFYC2_ARATH (1-115): 1.19e-50: 164: 26.09
+	At
		1285041 NFYB6_ARATH (8-179): 5.45e-66: 204: 40.81		NFYC1_ARATH (1-120): 1.36e-49: 162: 22.58
+	Hs
		670087 NFYB_HUMAN (1-207): 6.12e-93: 272: 100.00		DPOE4_HUMAN (10-84): 3.94e-24: 93.7: 32.00
+	At
		1319760 NFYB9_ARATH (3-197): 4.32e-64: 200: 34.67		NFYC2_ARATH (1-115): 1.19e-50: 164: 26.09
+	At
		1285036 NFYB6_ARATH (8-179): 5.45e-66: 204: 40.81		NFYC4_ARATH (1-120): 2.48e-47: 157: 23.02
+	At
		1285030 NFYB6_ARATH (8-179): 5.45e-66: 204: 40.81		NFYC9_ARATH (1-89): 8.78e-46: 153: 30.34
+	At
		1318055 NFYB9_ARATH (3-197): 4.32e-64: 200: 34.67		NFYC9_ARATH (1-89): 8.78e-46: 153: 30.34
+	At
		1285042 NFYB6_ARATH (8-179): 5.45e-66: 204: 40.81		NFYC3_ARATH (1-119): 6.41e-44: 147: 26.45
+	At
		1285035 NFYB6_ARATH (8-179): 5.45e-66: 204: 40.81		NFYC7_ARATH (1-109): 3.35e-43: 145: 28.70
+	At
		1285034 NFYB6_ARATH (8-179): 5.45e-66: 204: 40.81		NFYC6_ARATH (1-115): 5e-42: 142: 26.05
+	At
		1195758 NFYB6_ARATH (8-179): 5.45e-66: 204: 40.81		NFYC8_ARATH (1-109): 3.92e-41: 139: 23.68
+	At
		1285033 NFYB6_ARATH (8-179): 5.45e-66: 204: 40.81		NFYC5_ARATH (1-104): 6.27e-41: 139: 27.89
+	At
		1425056 NFYB9_ARATH (3-197): 4.32e-64: 200: 34.67		NFYC6_ARATH (1-115): 5e-42: 142: 26.05
+	At
		1195797 NFYB9_ARATH (3-197): 4.32e-64: 200: 34.67		NFYC8_ARATH (1-109): 3.92e-41: 139: 23.68
+	At
		1425050 NFYB9_ARATH (3-197): 4.32e-64: 200: 34.67		NFYC5_ARATH (1-104): 6.27e-41: 139: 27.89
+	At
		1201720 NFYB6_ARATH (8-179): 5.45e-66: 204: 40.81		NFYCA_ARATH (8-108): 1.14e-38: 133: 24.75
+	At
		1201733 NFYB9_ARATH (3-197): 4.32e-64: 200: 34.67		NFYCA_ARATH (8-108): 1.14e-38: 133: 24.75
+	At
		1285037 NFYB6_ARATH (8-179): 5.45e-66: 204: 40.81		DPB31_ARATH (9-89): 6.17e-24: 95.7: 24.69