HitPredict

Interactor 1: NUF2 (P33895)
Kinetochore protein NUF2
Gene: NUF2

Interactor 2: NDC80 (P40460)
Kinetochore protein NDC80 (80 kDa spindle component protein) (Nuclear division cycle protein 80) (Two-hybrid interaction with DMC1 protein 3)
Gene: NDC80

Species: Saccharomyces cerevisiae S288C

Source: mint

Category: Small-scale

Confidence: High

Method score Annotation score Interaction score
0.98 1.00 0.993

The method score is based on:

Property Details
Experiments 11087867, 11283351, 25230952, 14565975, 11782438, 11179222, 11266451, 24930965, 16554755, 16429126, 27156448, 25135934, 17634282, 18719252, 34313341, 36883282, 37968396, 38060647, 32997987, 20489023 and 8 others

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Identification Methods two hybrid, two hybrid pooling approach, pull down, affinity chromatography technology, fluorescent resonance energy transfer, biochemical, x-ray crystallography, anti tag coimmunoprecipitation, tandem affinity purification, chromatography technology
Interaction Type physical association, direct interaction, association

The annotation score is based on:

Property Details
Interacting Pfam domains DUF5595~Nuf2, Ndc80_HEC~Nuf2
Common GO terms GO:0000776 - kinetochore
GO:0000779 - condensed chromosome, centromeric region
GO:0005634 - nucleus
GO:0007059 - chromosome segregation
GO:0031262 - Ndc80 complex
and 2 others

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Homologous interactions 18

Homologous interactions

Color coding for hit scores

1 451
Sc

1 691
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Service provided by Combinatics, Japan

Last updated: 15 Jul 2024