HitPredict

Interactor 1: UB2D3 (P61077)
Ubiquitin-conjugating enzyme E2 D3 (EC 2.3.2.23) ((E3-independent) E2 ubiquitin-conjugating enzyme D3) (EC 2.3.2.24) (E2 ubiquitin-conjugating enzyme D3) (Ubiquitin carrier protein D3) (Ubiquitin-conjugating enzyme E2(17)KB 3) (Ubiquitin-conjugating enzyme E2-17 kDa 3) (Ubiquitin-protein ligase D3)
Gene: UBE2D3

Interactor 2: UBE4A (Q14139)
Ubiquitin conjugation factor E4 A (EC 2.3.2.27) (RING-type E3 ubiquitin transferase E4 A)
Gene: UBE4A

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.63	1.00	0.800

Property	Details
Experiments	19690564, 15611659
Identification Methods	two hybrid, pull down, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	U-box~UQ_con
Common GO terms	GO:0000151 - ubiquitin ligase complex GO:0000209 - protein polyubiquitination GO:0005634 - nucleus GO:0006511 - ubiquitin-dependent protein catabolic process
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 147 Hs			1 1066
+	Hs
		1048973 UB2D4_HUMAN (1-147): 8.97e-83: 241: 91.84		UBE4A_HUMAN (1-1066): 0: 1352: 100.00
+	Hs
		849328 UB2D1_HUMAN (1-147): 2.87e-82: 239: 88.44		UBE4A_HUMAN (1-1066): 0: 1352: 100.00
+	Hs
		919726 UB2D2_HUMAN (1-147): 4.16e-82: 239: 97.28		UBE4A_HUMAN (1-1066): 0: 1352: 100.00
+	Hs
		305165 UB2D2_HUMAN (1-147): 4.16e-82: 239: 97.28		UBE4B_HUMAN (145-1061): 0: 846: 25.50
+	Hs
		305164 UB2D3_HUMAN (1-147): 5.79e-81: 236: 100.00		UBE4B_HUMAN (145-1061): 0: 846: 25.50
+	Sc
		549827 UBC4_YEAST (3-147): 3.86e-79: 232: 80.00		UFD2_YEAST (148-1063): 0: 773: 22.81
+	Zm
		31012 B4FC97_MAIZE (1-147): 8.22e-83: 241: 78.91		A0A1D6KE36_MAIZE (955-1059): 3.31e-35: 145: 28.33
+	Ce
		105587 UBC2_CAEEL (1-147): 4.03e-82: 239: 92.52		G5ECY6_CAEEL (832-1065): 7.13e-30: 121: 17.87
+	At
		715129 UBC8_ARATH (1-147): 2.25e-82: 240: 79.59		CHIP_ARATH (941-1063): 2.11e-29: 120: 20.93
+	Os
		1014691 UBC5B_ORYSJ (1-147): 2.14e-81: 238: 78.91		PUB75_ORYSJ (954-1064): 4.21e-28: 121: 22.03
+	At
		715145 UBC9_ARATH (1-147): 6.25e-82: 239: 79.59		CHIP_ARATH (941-1063): 2.11e-29: 120: 20.93
+	At
		715154 UBC10_ARATH (1-147): 5.85e-82: 239: 78.91		CHIP_ARATH (941-1063): 2.11e-29: 120: 20.93
+	Hs
		1357485 UB2D4_HUMAN (1-147): 8.97e-83: 241: 91.84		CHIP_HUMAN (973-1060): 2.49e-27: 115: 33.71
+	Hs
		849489 UB2D1_HUMAN (1-147): 2.87e-82: 239: 88.44		CHIP_HUMAN (973-1060): 2.49e-27: 115: 33.71
+	Hs
		919907 UB2D2_HUMAN (1-147): 4.16e-82: 239: 97.28		CHIP_HUMAN (973-1060): 2.49e-27: 115: 33.71
+	Hs
		895956 UB2D3_HUMAN (1-147): 5.79e-81: 236: 100.00		CHIP_HUMAN (973-1060): 2.49e-27: 115: 33.71
+	Hs
		1297379 UB2E2_HUMAN (4-146): 1.76e-74: 222: 65.04		CHIP_HUMAN (973-1060): 2.49e-27: 115: 33.71
+	Hs
		1282365 UB2E3_HUMAN (4-146): 1.05e-73: 220: 65.73		CHIP_HUMAN (973-1060): 2.49e-27: 115: 33.71
+	Hs
		851908 UB2E1_HUMAN (4-146): 2.18e-72: 216: 65.04		CHIP_HUMAN (973-1060): 2.49e-27: 115: 33.71
+	At
		1339446 UBC29_ARATH (1-147): 7.96e-79: 231: 76.87		PUB29_ARATH (980-1054): 1.12e-13: 76.4: 34.21