HitPredict

Interactor 1: UBE2K (P61086)
Ubiquitin-conjugating enzyme E2 K (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme K) (Huntingtin-interacting protein 2) (HIP-2) (Ubiquitin carrier protein) (Ubiquitin-conjugating enzyme E2-25 kDa) (Ubiquitin-conjugating enzyme E2(25K)) (Ubiquitin-conjugating enzyme E2-25K) (Ubiquitin-protein ligase)
Gene: UBE2K

Interactor 2: RNF5 (Q99942)
E3 ubiquitin-protein ligase RNF5 (EC 2.3.2.27) (RING finger protein 5) (Ram1 homolog) (HsRma1)
Gene: RNF5

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.86	1.00	0.931

Property	Details
Experiments	25416956, 32296183, 19549727, unassigned1304, 32814053
Identification Methods	two hybrid, two hybrid prey pooling approach, two hybrid array, validated two hybrid, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-C3HC4_3
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0006511 - ubiquitin-dependent protein catabolic process GO:0070936 - protein K48-linked ubiquitination
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 200 Hs			1 180
+	Xl
		1140146 Q8AVM8_XENLA (1-200): 1.98e-82: 244: 97.00		Q5XGX4_XENLA (2-179): 3.83e-56: 176: 56.74
+	Hs
		896353 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		RN185_HUMAN (17-179): 7.02e-52: 166: 60.98
+	Hs
		1307272 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00
+	Hs
		849424 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00
+	Hs
		1292368 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		RN185_HUMAN (17-179): 7.02e-52: 166: 60.98
+	Hs
		849409 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		RN185_HUMAN (17-179): 7.02e-52: 166: 60.98
+	Hs
		896369 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		TRI31_HUMAN (19-126): 9.14e-27: 106: 20.91
+	Hs
		896381 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		TRI39_HUMAN (24-148): 5.72e-26: 104: 21.43
+	Hs
		849406 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		TRI11_HUMAN (14-148): 2.69e-31: 119: 17.78
+	Hs
		849314 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		TRI26_HUMAN (14-123): 1.73e-28: 112: 21.85
+	Hs
		896396 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		CBLC_HUMAN (6-93): 1.05e-19: 87.1: 28.28
+	Hs
		749302 UBE2K_HUMAN (1-200): 1.35e-82: 245: 100.00		BRCA1_HUMAN (25-76): 3.12e-19: 86.4: 34.62
+	Hs
		1318532 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI31_HUMAN (19-126): 9.14e-27: 106: 20.91
+	Hs
		1336390 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI39_HUMAN (24-148): 5.72e-26: 104: 21.43
+	Hs
		584907 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		RO52_HUMAN (23-148): 9.12e-25: 101: 18.11
+	Hs
		849366 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		TRI22_HUMAN (20-126): 2e-24: 100: 19.64
+	Hs
		1354526 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRI10_HUMAN (14-156): 5.89e-24: 99: 17.45
+	Hs
		262161 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		TRIM3_HUMAN (3-109): 3.92e-21: 91.3: 19.84
+	Hs
		262201 UB2D4_HUMAN (4-154): 1.49e-75: 225: 41.72		TRIM3_HUMAN (3-109): 3.92e-21: 91.3: 19.84
+	Hs
		749252 UB2D1_HUMAN (4-154): 1.87e-75: 225: 39.74		BRCA1_HUMAN (25-76): 3.12e-19: 86.4: 34.62