HitPredict

Interactor 1: RHOA (P61586)
Transforming protein RhoA (EC 3.6.5.2) (Rho cDNA clone 12) (h12)
Gene: RHOA

Interactor 2: RHG01 (Q07960)
Rho GTPase-activating protein 1 (CDC42 GTPase-activating protein) (GTPase-activating protein rhoGAP) (Rho-related small GTPase protein activator) (Rho-type GTPase-activating protein 1) (p50-RhoGAP)
Gene: ARHGAP1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.94	1.00	0.972

Property	Details
Experiments	16189514, 14749388, 9548756, 31871319, 9407060, 9535855, 20660160, 11927263, unassigned1913
Identification Methods	two hybrid, pull down, affinity chromatography technology, enzymatic study, x-ray crystallography, split luciferase complementation, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	Ras~RhoGAP
Common GO terms	GO:0005829 - cytosol GO:0007266 - Rho protein signal transduction GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 193 Hs			1 439
+	Mm
		1125742 RHOA_MOUSE (1-193): 4.1e-81: 240: 99.48		RHG01_MOUSE (1-439): 4.64e-163: 468: 96.58
+	Mm
		925791 RAC1_MOUSE (1-193): 8.86e-80: 237: 57.22		RHG01_MOUSE (1-439): 4.64e-163: 468: 96.58
+	Hs
		900564 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		RHG21_HUMAN (168-434): 3.18e-71: 246: 23.08
+	Hs
		900997 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		GMIP_HUMAN (171-437): 1.03e-68: 236: 22.57
+	Hs
		900429 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		RHG05_HUMAN (148-435): 5.93e-67: 233: 20.85
+	Hs
		900529 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		RHG31_HUMAN (234-439): 3.47e-66: 231: 25.81
+	Hs
		900634 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		RHG22_HUMAN (175-438): 7.18e-68: 230: 25.36
+	Hs
		901000 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		RHG23_HUMAN (179-438): 1.11e-65: 230: 25.19
+	Hs
		200213 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		SRGP3_HUMAN (182-434): 2.44e-66: 231: 26.44
+	Mm
		925805 RAC1_MOUSE (1-193): 8.86e-80: 237: 57.22		RHG22_MOUSE (175-438): 2.95e-67: 229: 25.36
+	Hs
		64681 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		RHG32_HUMAN (180-433): 7.32e-64: 225: 21.56
+	Hs
		64688 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		RHG32_HUMAN (180-433): 7.32e-64: 225: 21.56
+	Hs
		147862 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		RHG33_HUMAN (164-438): 1.47e-63: 224: 22.38
+	Mm
		925803 RAC1_MOUSE (1-193): 8.86e-80: 237: 57.22		RHG32_MOUSE (180-435): 3.64e-63: 222: 21.40
+	Hs
		900637 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		SRGP1_HUMAN (220-432): 6.87e-62: 218: 26.24
+	Mm
		925802 RAC1_MOUSE (1-193): 8.86e-80: 237: 57.22		CHIO_MOUSE (187-431): 1.39e-67: 220: 21.91
+	Hs
		302639 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		FA13A_HUMAN (242-431): 1.18e-25: 112: 34.03
+	Hs
		70663 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		MYO9A_HUMAN (235-420): 3.51e-23: 105: 32.45
+	Hs
		901006 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		RHG20_HUMAN (230-402): 5.91e-23: 103: 34.48
+	Hs
		900443 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		MYO9B_HUMAN (235-427): 3.06e-18: 89.7: 30.96