HitPredict

Interactor 1: RHOA (P61586)
Transforming protein RhoA (EC 3.6.5.2) (Rho cDNA clone 12) (h12)
Gene: RHOA

Interactor 2: SMUF1 (Q9HCE7)
E3 ubiquitin-protein ligase SMURF1 (hSMURF1) (EC 2.3.2.26) (HECT-type E3 ubiquitin transferase SMURF1) (SMAD ubiquitination regulatory factor 1) (SMAD-specific E3 ubiquitin-protein ligase 1)
Gene: SMURF1

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.93	0.60	0.750

Property	Details
Experiments	31623230, 16622418, 26825171, 28169289, 28294307, 28658604, 21708152, 15710384
Identification Methods	affinity chromatography technology, pull down, enzymatic study, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0060071 - Wnt signaling pathway, planar cell polarity pathway GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 193 Hs			1 757
+	Rn
		88544 RHOA_RAT (1-193): 4.1e-81: 240: 99.48		D3ZCF5_RAT (1-757): 0: 825: 93.53
+	Hs
		900923 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		SMUF2_HUMAN (1-757): 0: 794: 70.72
+	Mm
		56825 RAC1_MOUSE (1-193): 8.86e-80: 237: 57.22		SMUF2_MOUSE (1-757): 0: 795: 71.30
+	Hs
		900802 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		ITCH_HUMAN (1-757): 0: 733: 31.28
+	Hs
		925520 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		ITCH_HUMAN (1-757): 0: 733: 31.28
+	Hs
		436457 RHOC_HUMAN (1-193): 3.04e-78: 233: 91.71		ITCH_HUMAN (1-757): 0: 733: 31.28
+	Hs
		951237 RHOG_HUMAN (1-193): 2.83e-77: 231: 54.40		ITCH_HUMAN (1-757): 0: 733: 31.28
+	Hs
		894313 CDC42_HUMAN (1-193): 5.51e-77: 230: 52.33		ITCH_HUMAN (1-757): 0: 733: 31.28
+	Hs
		545560 RAC2_HUMAN (1-193): 3.9e-76: 228: 53.61		ITCH_HUMAN (1-757): 0: 733: 31.28
+	Hs
		892540 RAC3_HUMAN (1-179): 4.85e-74: 223: 57.54		ITCH_HUMAN (1-757): 0: 733: 31.28
+	Hs
		125308 RHOA_HUMAN (1-193): 1.4e-80: 239: 100.00		WWP2_HUMAN (97-757): 0: 708: 36.69
+	Hs
		125349 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		WWP2_HUMAN (97-757): 0: 708: 36.69
+	Hs
		125100 RHOC_HUMAN (1-193): 3.04e-78: 233: 91.71		WWP2_HUMAN (97-757): 0: 708: 36.69
+	Hs
		125372 RHOG_HUMAN (1-193): 2.83e-77: 231: 54.40		WWP2_HUMAN (97-757): 0: 708: 36.69
+	Hs
		125298 CDC42_HUMAN (1-193): 5.51e-77: 230: 52.33		WWP2_HUMAN (97-757): 0: 708: 36.69
+	Hs
		125135 RAC2_HUMAN (1-193): 3.9e-76: 228: 53.61		WWP2_HUMAN (97-757): 0: 708: 36.69
+	Hs
		125294 RAC3_HUMAN (1-179): 4.85e-74: 223: 57.54		WWP2_HUMAN (97-757): 0: 708: 36.69
+	Hs
		125330 RHOB_HUMAN (1-191): 1.62e-73: 221: 83.94		WWP2_HUMAN (97-757): 0: 708: 36.69
+	Hs
		808080 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		NEDD4_HUMAN (93-755): 0: 673: 36.63
+	Hs
		925397 RAC1_HUMAN (1-193): 8.86e-80: 237: 57.22		HUWE1_HUMAN (71-756): 0: 583: 30.14