HitPredict

Interactor 1: 1433E (P62258)
14-3-3 protein epsilon (14-3-3E)
Gene: YWHAE

Interactor 2: M3K21 (Q5TCX8)
Mitogen-activated protein kinase kinase kinase 21 (EC 2.7.11.25) (Mitogen-activated protein kinase kinase kinase MLK4) (Mixed lineage kinase 4)
Gene: MAP3K21

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.76	1.00	0.876

Property	Details
Experiments	32707033, 35271311, 36931259
Identification Methods	affinity chromatography technology, proximity labelling technology, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	14-3-3~PK_Tyr_Ser-Thr
Common GO terms	GO:0005737 - cytoplasm GO:0007165 - signal transduction
Homologous interactions	10

Homologous interactions

Color coding for hit scores

	1 255 Hs			1 1036
+	Hs
		909462 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		M3K10_HUMAN (32-1021): 0: 570: 44.87
+	Hs
		909438 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		M3K9_HUMAN (38-1036): 0: 561: 47.56
+	Hs
		910094 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		M3K20_HUMAN (117-932): 2.36e-131: 419: 21.37
+	Hs
		909810 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		MINK1_HUMAN (104-1027): 1.61e-122: 408: 15.40
+	Hs
		337416 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		ABL1_HUMAN (68-887): 6.57e-122: 402: 18.07
+	Hs
		910013 1433E_HUMAN (1-255): 4.87e-154: 430: 100.00		SIK2_HUMAN (124-1029): 1.57e-119: 391: 17.95
+	At
		965327 14332_ARATH (4-255): 1.05e-147: 414: 74.41		PBS1_ARATH (61-498): 1.57e-131: 407: 20.22
+	At
		1277300 14337_ARATH (2-255): 7.73e-147: 412: 71.21		SERK1_ARATH (1-443): 8.68e-128: 403: 18.97
+	At
		965320 14332_ARATH (4-255): 1.05e-147: 414: 74.41		BAK1_ARATH (1-450): 1.24e-126: 400: 19.03
+	At
		788521 14333_ARATH (1-253): 3.45e-147: 413: 74.12		PBL9_ARATH (57-403): 1.03e-123: 385: 20.11