HitPredict

Interactor 1: ARF6 (P62330)
ADP-ribosylation factor 6 (EC 3.6.5.2)
Gene: ARF6

Interactor 2: GGA3 (Q9NZ52)
ADP-ribosylation factor-binding protein GGA3 (Golgi-localized, gamma ear-containing, ARF-binding protein 3)
Gene: GGA3

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.87	1.00	0.935

Property	Details
Experiments	19327867, 17804820, 20848231, 18199687, 23143332, 24056087
Identification Methods	pull down, affinity chromatography technology
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	Arf~GGA_N-GAT
Common GO terms	GO:0005794 - Golgi apparatus GO:0006886 - intracellular protein transport GO:0031901 - early endosome membrane GO:0034394 - protein localization to cell surface GO:0055038 - recycling endosome membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 175 Hs			1 723
+	Hs
		950568 ARF1_HUMAN (2-175): 4.43e-69: 209: 68.39		GGA3_HUMAN (1-723): 3.49e-148: 451: 100.00
+	Hs
		897409 ARF3_HUMAN (2-175): 4.91e-65: 198: 69.54		GGA3_HUMAN (1-723): 3.49e-148: 451: 100.00
+	Mm
		950589 ARF1_MOUSE (2-175): 4.43e-69: 209: 68.39		GGA3_MOUSE (1-723): 5.14e-130: 403: 81.27
+	Rn
		88619 ARF1_RAT (2-175): 4.43e-69: 209: 68.39		D3ZHG8_RAT (1-723): 6.55e-121: 378: 67.08
+	Rn
		88618 ARF6_RAT (1-175): 2.17e-65: 199: 100.00		D3ZHG8_RAT (1-723): 6.55e-121: 378: 67.08
+	Rn
		88617 ARF3_RAT (2-175): 4.91e-65: 198: 69.54		D3ZHG8_RAT (1-723): 6.55e-121: 378: 67.08
+	Hs
		950575 ARF1_HUMAN (2-175): 4.43e-69: 209: 68.39		GGA1_HUMAN (2-723): 1.09e-91: 301: 39.78
+	Hs
		950740 ARF5_HUMAN (2-173): 4.69e-69: 209: 65.12		GGA1_HUMAN (2-723): 1.09e-91: 301: 39.78
+	Hs
		913175 ARF6_HUMAN (1-175): 2.17e-65: 199: 100.00		GGA1_HUMAN (2-723): 1.09e-91: 301: 39.78
+	Hs
		897416 ARF3_HUMAN (2-175): 4.91e-65: 198: 69.54		GGA1_HUMAN (2-723): 1.09e-91: 301: 39.78
+	Hs
		277049 ARF5_HUMAN (2-173): 4.69e-69: 209: 65.12		STAM2_HUMAN (1-480): 4.23e-79: 264: 16.95
+	Dm
		778418 ARF6_DROME (1-174): 4e-68: 206: 97.13		Q9W329_DROME (9-705): 8.66e-69: 240: 22.06
+	Sc
		503143 ARF1_YEAST (2-175): 2.05e-68: 207: 66.09		GGA1_YEAST (2-425): 1.01e-68: 237: 21.28
+	Sc
		503089 ARF1_YEAST (2-175): 2.05e-68: 207: 66.09		GGA2_YEAST (2-437): 2.07e-66: 231: 21.70
+	Hs
		212618 ARF1_HUMAN (2-175): 4.43e-69: 209: 68.39		AP1G1_HUMAN (503-720): 2.89e-51: 193: 26.96
+	Hs
		212615 ARF6_HUMAN (1-175): 2.17e-65: 199: 100.00		AP1G1_HUMAN (503-720): 2.89e-51: 193: 26.96
+	Hs
		574772 ARF4_HUMAN (1-173): 1.32e-70: 212: 64.74		GGA2_HUMAN (456-721): 1.1e-25: 114: 34.83
+	Hs
		950574 ARF1_HUMAN (2-175): 4.43e-69: 209: 68.39		GGA2_HUMAN (456-721): 1.1e-25: 114: 34.83
+	Hs
		913174 ARF6_HUMAN (1-175): 2.17e-65: 199: 100.00		GGA2_HUMAN (456-721): 1.1e-25: 114: 34.83
+	Hs
		897415 ARF3_HUMAN (2-175): 4.91e-65: 198: 69.54		GGA2_HUMAN (456-721): 1.1e-25: 114: 34.83