HitPredict

Interactor 1: RAC1 (P63000)
Ras-related C3 botulinum toxin substrate 1 (EC 3.6.5.2) (Cell migration-inducing gene 5 protein) (Ras-like protein TC25) (p21-Rac1)
Gene: RAC1

Interactor 2: GDIR3 (Q99819)
Rho GDP-dissociation inhibitor 3 (Rho GDI 3) (Rho-GDI gamma)
Gene: ARHGDIG

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	1.00	0.691

Property	Details
Experiments	35271311
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Ras~Rho_GDI
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0016020 - membrane GO:0031410 - cytoplasmic vesicle
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 192 Hs			1 225
+	Hs
		951270 RHOG_HUMAN (1-192): 3.1e-79: 236: 71.35		GDIR3_HUMAN (1-225): 1.49e-115: 330: 100.00
+	Hs
		894357 CDC42_HUMAN (1-192): 1.4e-78: 234: 70.31		GDIR3_HUMAN (1-225): 1.49e-115: 330: 100.00
+	Hs
		900840 RHOA_HUMAN (5-192): 3.5e-77: 230: 57.98		GDIR3_HUMAN (1-225): 1.49e-115: 330: 100.00
+	Mm
		925816 RAC1_MOUSE (1-192): 3.81e-86: 253: 100.00		GDIR1_MOUSE (36-225): 6.84e-105: 302: 60.53
+	Hs
		854769 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		GDIR1_HUMAN (36-225): 1.46e-104: 301: 61.05
+	Hs
		545278 RAC2_HUMAN (1-192): 1.2e-82: 244: 92.19		GDIR1_HUMAN (36-225): 1.46e-104: 301: 61.05
+	Mm
		892716 RAC3_MOUSE (1-191): 2.72e-79: 236: 93.19		GDIR1_MOUSE (36-225): 6.84e-105: 302: 60.53
+	Hs
		854759 RAC3_HUMAN (1-191): 2.72e-79: 236: 93.19		GDIR1_HUMAN (36-225): 1.46e-104: 301: 61.05
+	Hs
		854771 RHOG_HUMAN (1-192): 3.1e-79: 236: 71.35		GDIR1_HUMAN (36-225): 1.46e-104: 301: 61.05
+	Hs
		854760 CDC42_HUMAN (1-192): 1.4e-78: 234: 70.31		GDIR1_HUMAN (36-225): 1.46e-104: 301: 61.05
+	Hs
		854851 RAC1_HUMAN (1-192): 3.81e-86: 253: 100.00		GDIR2_HUMAN (47-225): 5.49e-96: 280: 60.89
+	Rn
		901290 RHOA_RAT (5-192): 1.43e-77: 231: 57.98		GDIR1_RAT (36-225): 6.84e-105: 302: 60.53
+	Hs
		854763 RHOA_HUMAN (5-192): 3.5e-77: 230: 57.98		GDIR1_HUMAN (36-225): 1.46e-104: 301: 61.05
+	Mm
		892713 RAC3_MOUSE (1-191): 2.72e-79: 236: 93.19		GDIR2_MOUSE (27-225): 2.17e-101: 293: 56.22
+	Hs
		545279 RAC2_HUMAN (1-192): 1.2e-82: 244: 92.19		GDIR2_HUMAN (47-225): 5.49e-96: 280: 60.89
+	Hs
		854847 RAC3_HUMAN (1-191): 2.72e-79: 236: 93.19		GDIR2_HUMAN (47-225): 5.49e-96: 280: 60.89
+	Hs
		854852 RHOG_HUMAN (1-192): 3.1e-79: 236: 71.35		GDIR2_HUMAN (47-225): 5.49e-96: 280: 60.89
+	At
		1105170 RAC5_ARATH (4-177): 1.11e-79: 237: 64.94		GDIR_ARATH (27-223): 4.23e-80: 241: 28.64
+	Ce
		975573 RAC1_CAEEL (1-192): 2.2e-83: 246: 82.81		GDIR_CAEEL (47-222): 9.12e-76: 228: 37.29
+	Cl
		47354 RAC1_CANLF (1-192): 3.81e-86: 253: 100.00		A0A8I3N6C6_CANLF (36-225): 9.11e-70: 212: 44.21