HitPredict

Interactor 1: SMAD3 (P84022)
Mothers against decapentaplegic homolog 3 (MAD homolog 3) (Mad3) (Mothers against DPP homolog 3) (hMAD-3) (JV15-2) (SMAD family member 3) (SMAD 3) (Smad3) (hSMAD3)
Gene: SMAD3

Interactor 2: ITCH (Q96J02)
E3 ubiquitin-protein ligase Itchy homolog (Itch) (EC 2.3.2.26) (Atrophin-1-interacting protein 4) (AIP4) (HECT-type E3 ubiquitin transferase Itchy homolog) (NFE2-associated polypeptide 1) (NAPP1)
Gene: ITCH

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.60	0.60	0.602

Property	Details
Experiments	37219487, 15051726
Identification Methods	unspecified method, pull down, phage display
Interaction Type	direct interaction

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0043066 - negative regulation of apoptotic process
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 425 Hs			1 903
+	Hs
		1078624 SMAD2_HUMAN (1-425): 0: 589: 83.73		ITCH_HUMAN (1-903): 0: 926: 100.00
+	Mm
		1143948 SMAD2_MOUSE (1-425): 0: 591: 83.73		ITCH_MOUSE (1-903): 0: 885: 91.16
+	Hs
		950278 SMAD3_HUMAN (1-425): 0: 636: 100.00		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		125369 SMAD3_HUMAN (1-425): 0: 636: 100.00		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		1078654 SMAD2_HUMAN (1-425): 0: 589: 83.73		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Hs
		125487 SMAD2_HUMAN (1-425): 0: 589: 83.73		WWP2_HUMAN (1-903): 0: 816: 54.08
+	Hs
		950249 SMAD3_HUMAN (1-425): 0: 636: 100.00		NED4L_HUMAN (5-900): 0: 762: 32.14
+	Hs
		1078757 SMAD1_HUMAN (1-425): 0: 559: 60.09		WWP1_HUMAN (1-903): 0: 832: 57.54
+	Dm
		785516 MAD_DROME (1-425): 0: 571: 61.09		SUDX_DROME (19-903): 0: 788: 48.29
+	Hs
		1078629 SMAD2_HUMAN (1-425): 0: 589: 83.73		NED4L_HUMAN (5-900): 0: 762: 32.14
+	Hs
		808087 SMAD3_HUMAN (1-425): 0: 636: 100.00		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		808134 SMAD2_HUMAN (1-425): 0: 589: 83.73		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		808135 SMAD1_HUMAN (1-425): 0: 559: 60.09		NEDD4_HUMAN (90-900): 0: 673: 34.16
+	Hs
		950291 SMAD3_HUMAN (1-425): 0: 636: 100.00		SMUF1_HUMAN (1-535): 1.85e-47: 183: 14.71
+	Hs
		950287 SMAD3_HUMAN (1-425): 0: 636: 100.00		SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94
+	Hs
		1078671 SMAD2_HUMAN (1-425): 0: 589: 83.73		SMUF1_HUMAN (1-535): 1.85e-47: 183: 14.71
+	Hs
		1078667 SMAD2_HUMAN (1-425): 0: 589: 83.73		SMUF2_HUMAN (1-358): 2.56e-46: 180: 16.94
+	Mm
		56826 SMAD1_MOUSE (1-425): 0: 560: 59.87		SMUF2_MOUSE (1-358): 2.99e-46: 179: 16.67
+	Dm
		785477 MAD_DROME (1-425): 0: 571: 61.09		SMUF1_DROME (16-518): 1.15e-39: 161: 16.19
+	Mm
		936618 SMAD1_MOUSE (1-425): 0: 560: 59.87		SMUF1_MOUSE (1-535): 3.62e-41: 164: 14.07