HitPredict

Interactor 1: SMAD3 (P84022)
Mothers against decapentaplegic homolog 3 (MAD homolog 3) (Mad3) (Mothers against DPP homolog 3) (hMAD-3) (JV15-2) (SMAD family member 3) (SMAD 3) (Smad3) (hSMAD3)
Gene: SMAD3

Interactor 2: CHRD (Q9H2X0)
Chordin
Gene: CHRD

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.54	0.60	0.573

Property	Details
Experiments	21988832
Identification Methods	affinity chromatography technology, two hybrid, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0045668 - negative regulation of osteoblast differentiation
Homologous interactions	12

Homologous interactions

Color coding for hit scores

	1 425 Hs			1 955
+	Hs
		358494 SMAD3_HUMAN (1-425): 0: 636: 100.00		CO1A1_HUMAN (689-767): 4.68e-09: 62.6: 28.75
+	Mm
		435996 SMAD3_MOUSE (1-425): 0: 636: 100.00		CO3A1_MOUSE (701-771): 5.36e-09: 62.6: 28.77
+	Mm
		435995 SMAD2_MOUSE (1-425): 0: 591: 83.73		CO3A1_MOUSE (701-771): 5.36e-09: 62.6: 28.77
+	Hs
		172669 SMAD2_HUMAN (1-425): 0: 589: 83.73		LAMA5_HUMAN (589-784): 4.99e-09: 62.9: 13.85
+	Hs
		358519 SMAD2_HUMAN (1-425): 0: 589: 83.73		CO1A1_HUMAN (689-767): 4.68e-09: 62.6: 28.75
+	Hs
		980382 SMAD1_HUMAN (1-425): 0: 559: 60.09		NOTC2_HUMAN (575-913): 9.96e-16: 84.5: 10.43
+	Mm
		435992 SMAD1_MOUSE (1-425): 0: 560: 59.87		CO3A1_MOUSE (701-771): 5.36e-09: 62.6: 28.77
+	Hs
		1035611 SMAD4_HUMAN (23-414): 1.55e-117: 355: 33.21		TEN3_HUMAN (545-744): 3.06e-13: 76.4: 10.57
+	Mm
		435994 SMAD4_MOUSE (23-414): 2.27e-119: 360: 32.88		CO3A1_MOUSE (701-771): 5.36e-09: 62.6: 28.77
+	Hs
		877409 SMAD4_HUMAN (23-414): 1.55e-117: 355: 33.21		LAMB2_HUMAN (547-779): 2.93e-09: 63.3: 11.20
+	Hs
		358510 SMAD4_HUMAN (23-414): 1.55e-117: 355: 33.21		CO1A1_HUMAN (689-767): 4.68e-09: 62.6: 28.75
+	Mm
		192480 SMAD7_MOUSE (28-403): 8.69e-99: 303: 27.06		CO3A1_MOUSE (701-771): 5.36e-09: 62.6: 28.77