HitPredict

Interactor 1: SMAD4 (Q13485)
Mothers against decapentaplegic homolog 4 (MAD homolog 4) (Mothers against DPP homolog 4) (Deletion target in pancreatic carcinoma 4) (SMAD family member 4) (SMAD 4) (Smad4) (hSMAD4)
Gene: SMAD4

Interactor 2: FBW1A (Q9Y297)
F-box/WD repeat-containing protein 1A (E3RSIkappaB) (Epididymis tissue protein Li 2a) (F-box and WD repeats protein beta-TrCP) (pIkappaBalpha-E3 receptor subunit)
Gene: BTRC

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.92	0.60	0.747

Property	Details
Experiments	26022109, 14988407, 16865698, 15855639, 25373906, 33199824, 15314162
Identification Methods	affinity chromatography technology, two hybrid, anti tag coimmunoprecipitation, anti bait coimmunoprecipitation
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0045892 - negative regulation of DNA-templated transcription GO:0045893 - positive regulation of DNA-templated transcription
Homologous interactions	5

Homologous interactions

Color coding for hit scores

	1 552 Hs			1 605
+	Mm
		956539 SMAD4_MOUSE (1-552): 0: 688: 98.37		FBW1A_MOUSE (1-605): 0: 547: 98.68
+	Hs
		950332 SMAD3_HUMAN (19-549): 1.96e-176: 506: 31.22		FBW1A_HUMAN (1-605): 0: 551: 100.00
+	Hs
		1078740 SMAD1_HUMAN (20-546): 0: 542: 34.91		FBXW7_HUMAN (76-527): 7.38e-83: 276: 23.71
+	Dm
		785459 MAD_DROME (20-546): 3.27e-175: 504: 29.89		LIS1_DROME (24-411): 2.97e-53: 189: 14.39
+	Hs
		167390 SMAD7_HUMAN (33-137): 4.06e-26: 112: 28.45		TBL1R_HUMAN (501-605): 3.85e-15: 80.4: 16.67