HitPredict

Interactor 1: AGAP2 (Q3UHD9)
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 (AGAP-2) (Centaurin-gamma-1) (Cnt-g1) (Phosphatidylinositol 3-kinase enhancer) (PIKE)
Gene: AGAP2

Interactor 2: SHAN3 (Q4ACU6)
SH3 and multiple ankyrin repeat domains protein 3 (Shank3) (Proline-rich synapse-associated protein 2) (ProSAP2) (SPANK-2)
Gene: Shank3

Species: Mus musculus

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.861

Property	Details
Experiments	28706196, 27507650, 28671696
Identification Methods	affinity chromatography technology, anti bait coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Ank_2~Ank_2, Ank_2~ArfGap, Ank_2~Ras
Common GO terms	GO:0005737 - cytoplasm GO:0035255 - ionotropic glutamate receptor binding GO:0044877 - protein-containing complex binding GO:0098978 - glutamatergic synapse
Homologous interactions	9

Homologous interactions

Color coding for hit scores

	1 1186 Mm			1 1730
+	Mm
		1112608 AGAP2_MOUSE (1-1186): 0: 1024: 100.00		SHAN2_MOUSE (352-1727): 1.03e-90: 328: 35.55
+	Mm
		103599 AGAP2_MOUSE (1-1186): 0: 1024: 100.00		ANK3_MOUSE (41-592): 7.79e-85: 311: 15.58
+	Mm
		88367 AGAP2_MOUSE (1-1186): 0: 1024: 100.00		SHAN1_MOUSE (367-1725): 5.86e-28: 126: 14.36
+	Mm
		1112622 AGAP2_MOUSE (1-1186): 0: 1024: 100.00		ANK2_MOUSE (134-335): 6.85e-09: 63.5: 31.19
+	Hs
		1138562 AGAP7_HUMAN (528-1145): 7.03e-152: 472: 41.44		SHAN3_HUMAN (1-1730): 0: 601: 95.21
+	Mm
		1115948 GIT1_MOUSE (932-1139): 6.25e-72: 258: 32.88		SHAN3_MOUSE (1-1730): 0: 626: 100.00
+	Mm
		919692 RAP1A_MOUSE (398-567): 1.81e-70: 235: 23.03		SHAN3_MOUSE (1-1730): 0: 626: 100.00
+	Hs
		1050179 GIT2_HUMAN (932-1139): 1.58e-73: 262: 31.98		PCLO_HUMAN (347-1361): 2.55e-17: 91.3: 10.90
+	Hs
		1377463 GIT1_HUMAN (932-1139): 4.84e-72: 258: 32.88		PCLO_HUMAN (347-1361): 2.55e-17: 91.3: 10.90