HitPredict

Interactor 1: OTUB1 (Q96FW1)
Ubiquitin thioesterase OTUB1 (EC 3.4.19.12) (Deubiquitinating enzyme OTUB1) (OTU domain-containing ubiquitin aldehyde-binding protein 1) (Otubain-1) (hOTU1) (Ubiquitin-specific-processing protease OTUB1)
Gene: OTUB1

Interactor 2: UB2D4 (Q9Y2X8)
Ubiquitin-conjugating enzyme E2 D4 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme D4) (HBUCE1) (Ubiquitin carrier protein D4) (Ubiquitin-protein ligase D4)
Gene: UBE2D4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.86	1.00	0.931

Property	Details
Experiments	25416956, 32296183, 33961781, 31515488, unassigned1304
Identification Methods	two hybrid, affinity chromatography technology, two hybrid array, validated two hybrid, two hybrid prey pooling approach, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	Peptidase_C65~UQ_con
Common GO terms	GO:0031625 - ubiquitin protein ligase binding
Homologous interactions	10

Homologous interactions

Color coding for hit scores

	1 271 Hs			1 147
+	Hs
		849408 OTUB1_HUMAN (1-271): 3.25e-150: 422: 100.00		UB2D1_HUMAN (1-147): 4.61e-83: 241: 91.84
+	Hs
		919824 OTUB1_HUMAN (1-271): 3.25e-150: 422: 100.00		UB2D2_HUMAN (1-147): 1.54e-82: 240: 93.20
+	Hs
		895878 OTUB1_HUMAN (1-271): 3.25e-150: 422: 100.00		UB2D3_HUMAN (1-147): 1.56e-81: 238: 91.84
+	Hs
		1291545 OTUB1_HUMAN (1-271): 3.25e-150: 422: 100.00		UB2E2_HUMAN (4-146): 3.5e-75: 224: 64.34
+	Hs
		1282326 OTUB1_HUMAN (1-271): 3.25e-150: 422: 100.00		UB2E3_HUMAN (4-146): 1.24e-74: 223: 64.34
+	Hs
		851868 OTUB1_HUMAN (1-271): 3.25e-150: 422: 100.00		UB2E1_HUMAN (4-146): 4.67e-73: 218: 64.34
+	Hs
		896520 OTUB1_HUMAN (1-271): 3.25e-150: 422: 100.00		UBE2N_HUMAN (4-147): 1.52e-69: 208: 46.53
+	Mm
		896585 OTUB1_MOUSE (1-271): 3.18e-150: 422: 99.63		UBE2N_MOUSE (4-147): 1.52e-69: 208: 46.53
+	Ce
		715115 OTUBL_CAEEL (3-271): 2.76e-122: 352: 34.42		UBC2_CAEEL (1-147): 1.59e-82: 240: 92.52
+	Hs
		896513 OTUB2_HUMAN (47-270): 1.25e-104: 305: 47.77		UBE2N_HUMAN (4-147): 1.52e-69: 208: 46.53