HitPredict

Interactor 1: CHM4 (Q8T0Q4)
Charged multivesicular body protein 4 (CHMP4) (ESCRT-III complex core component shrb) (Protein Shrub)
Gene: shrb

Interactor 2: CHMP5 (Q9VVI9)
Charged multivesicular body protein 5 (Vacuolar protein-sorting-associated protein 60)
Gene: Vps60

Species: Drosophila melanogaster

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.54	1.00	0.737

Property	Details
Experiments	22036573, 38944040
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Snf7~Snf7
Common GO terms	GO:0000815 - ESCRT III complex GO:0005771 - multivesicular body GO:0006900 - vesicle budding from membrane GO:0032509 - endosome transport via multivesicular body sorting pathway GO:0032511 - late endosome to vacuole transport via multivesicular body sorting pathway and 1 others more... GO:0046718 - symbiont entry into host cell
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 226 Dm			1 226
+	Hs
		97297 E9PSI1_HUMAN (1-202): 7.68e-57: 194: 55.07		CHM4B_HUMAN (15-218): 3.53e-25: 100: 23.42
+	Ce
		195474 Q18886_CAEEL (1-226): 4.85e-47: 156: 55.51		O16458_CAEEL (1-221): 5.74e-35: 125: 54.51
+	Dm
		1345389 CHM4_DROME (1-226): 7.68e-50: 163: 100.00		CHM4_DROME (7-222): 1.35e-30: 114: 24.42
+	Ce
		1166323 Q18886_CAEEL (1-226): 4.85e-47: 156: 55.51		Q9U2F6_CAEEL (5-208): 8.11e-32: 117: 18.63
+	Dm
		1345400 CHM4_DROME (1-226): 7.68e-50: 163: 100.00		Q9VN02_DROME (4-219): 4.79e-29: 110: 12.96
+	Zm
		80572 B4FU00_MAIZE (4-213): 5.7e-39: 136: 20.74		B4FU00_MAIZE (1-222): 5.01e-38: 133: 36.28
+	Hs
		1357273 CHM4C_HUMAN (1-226): 2.9e-42: 144: 48.50		CHMP7_HUMAN (5-222): 2.96e-32: 123: 19.91
+	Dm
		1345394 CHM4_DROME (1-226): 7.68e-50: 163: 100.00		Q9VBI3_DROME (7-208): 7.07e-25: 100: 17.82
+	Dm
		1345401 CHM4_DROME (1-226): 7.68e-50: 163: 100.00		Q9VRJ5_DROME (2-211): 2.75e-25: 100: 13.33
+	Dm
		1345410 CHM4_DROME (1-226): 7.68e-50: 163: 100.00		Q9W236_DROME (1-209): 2.87e-22: 92.2: 20.28
+	Hs
		1385817 CHM4C_HUMAN (1-226): 2.9e-42: 144: 48.50		CHM4C_HUMAN (6-218): 1.52e-26: 104: 20.28
+	Hs
		1385854 CHM4C_HUMAN (1-226): 2.9e-42: 144: 48.50		CHM4A_HUMAN (1-219): 7.45e-26: 101: 22.73
+	At
		307755 VP601_ARATH (4-217): 7.34e-40: 138: 19.91		VP321_ARATH (1-223): 2.97e-28: 107: 27.35
+	Hs
		1385865 CHM4C_HUMAN (1-226): 2.9e-42: 144: 48.50		CHM4B_HUMAN (15-218): 3.53e-25: 100: 23.42
+	At
		102954 F4JXR3_ARATH (4-161): 4.69e-40: 140: 20.61		VP322_ARATH (1-223): 2.5e-25: 100: 27.11
+	At
		1442471 VP601_ARATH (4-217): 7.34e-40: 138: 19.91		VP322_ARATH (1-223): 2.5e-25: 100: 27.11
+	At
		1082311 VP601_ARATH (4-217): 7.34e-40: 138: 19.91		VPS2B_ARATH (7-219): 3.83e-25: 99.8: 15.96
+	At
		1082317 VPS2A_ARATH (3-219): 6.18e-39: 136: 16.51		VPS2B_ARATH (7-219): 3.83e-25: 99.8: 15.96
+	Mm
		1424143 CHM4B_MOUSE (1-225): 1.08e-38: 135: 56.96		CHM4B_MOUSE (15-218): 2.24e-25: 100: 23.42