HitPredict

Interactor 1: CHM4 (Q8T0Q4)
Charged multivesicular body protein 4 (CHMP4) (ESCRT-III complex core component shrb) (Protein Shrub)
Gene: shrb

Interactor 2: Q9VN02 (Q9VN02)
GH14561p (Vacuolar protein sorting 24, isoform A) (Vacuolar protein sorting 24, isoform B)
Gene: Vps24

Species: Drosophila melanogaster

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.69	1.00	0.835

Property	Details
Experiments	14605208, 22036573, 38944040
Identification Methods	two hybrid, affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	Snf7~Snf7
Common GO terms	GO:0000815 - ESCRT III complex GO:0005771 - multivesicular body GO:0032509 - endosome transport via multivesicular body sorting pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 226 Dm			1 223
+	Ce
		1166323 Q18886_CAEEL (1-226): 4.85e-47: 156: 55.51		Q9U2F6_CAEEL (3-222): 5.52e-52: 169: 23.50
+	Dm
		1345401 CHM4_DROME (1-226): 7.68e-50: 163: 100.00		Q9VRJ5_DROME (2-209): 5.43e-45: 151: 27.40
+	Dm
		1345394 CHM4_DROME (1-226): 7.68e-50: 163: 100.00		Q9VBI3_DROME (3-202): 2.95e-43: 147: 23.50
+	Ce
		1166321 Q18886_CAEEL (1-226): 4.85e-47: 156: 55.51		Q9GYK4_CAEEL (1-211): 1.15e-44: 149: 49.76
+	Hs
		1433142 CHM4B_HUMAN (1-225): 1.24e-38: 134: 56.96		CHMP3_HUMAN (1-223): 9.55e-53: 171: 58.74
+	At
		1082311 VP601_ARATH (4-217): 7.34e-40: 138: 19.91		VPS2B_ARATH (1-222): 4.52e-50: 164: 24.32
+	Hs
		1453653 CHMP5_HUMAN (4-200): 4.32e-37: 131: 22.06		CHMP3_HUMAN (1-223): 9.55e-53: 171: 58.74
+	At
		1082317 VPS2A_ARATH (3-219): 6.18e-39: 136: 16.51		VPS2B_ARATH (1-222): 4.52e-50: 164: 24.32
+	Dm
		1345391 CHM4_DROME (1-226): 7.68e-50: 163: 100.00		Q95SH2_DROME (23-222): 3.93e-38: 132: 16.50
+	At
		102953 F4JXR3_ARATH (4-161): 4.69e-40: 140: 20.61		VP241_ARATH (7-223): 9.62e-46: 153: 40.72
+	At
		1424667 VP601_ARATH (4-217): 7.34e-40: 138: 19.91		VP241_ARATH (7-223): 9.62e-46: 153: 40.72
+	Ce
		1166322 Q18886_CAEEL (1-226): 4.85e-47: 156: 55.51		Q9TXI3_CAEEL (22-223): 3.42e-38: 133: 13.79
+	At
		1424668 VPS2A_ARATH (3-219): 6.18e-39: 136: 16.51		VP241_ARATH (7-223): 9.62e-46: 153: 40.72
+	Hs
		221882 CHM4B_HUMAN (1-225): 1.24e-38: 134: 56.96		CHM2A_HUMAN (3-222): 1.37e-45: 152: 22.73
+	Hs
		1433130 CHM4B_HUMAN (1-225): 1.24e-38: 134: 56.96		CHM2B_HUMAN (9-204): 7.49e-45: 150: 22.45
+	Hs
		221888 CHMP5_HUMAN (4-200): 4.32e-37: 131: 22.06		CHM2A_HUMAN (3-222): 1.37e-45: 152: 22.73
+	Hs
		1433081 CHM4B_HUMAN (1-225): 1.24e-38: 134: 56.96		CHM1A_HUMAN (22-223): 2.67e-37: 130: 11.39
+	Hs
		1439645 CHMP5_HUMAN (4-200): 4.32e-37: 131: 22.06		CHM1A_HUMAN (22-223): 2.67e-37: 130: 11.39
+	Hs
		1273179 CHM4B_HUMAN (1-225): 1.24e-38: 134: 56.96		CHM1B_HUMAN (23-223): 2.59e-35: 125: 13.93
+	Hs
		1273184 CHMP5_HUMAN (4-200): 4.32e-37: 131: 22.06		CHM1B_HUMAN (23-223): 2.59e-35: 125: 13.93