HitPredict

Interactor 1: ASAP2 (O43150)
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 (Development and differentiation-enhancing factor 2) (Paxillin-associated protein with ARF GAP activity 3) (PAG3) (Pyk2 C-terminus-associated protein) (PAP)
Gene: ASAP2

Interactor 2: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.861

Property	Details
Experiments	26186194, 28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Ank_2~SH3_1, Ank_2~SH3_9, PH~SH3_1, PH~SH3_9, SH3_9~SH3_9
Common GO terms	GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1006 Hs			1 380
+	Hs
		195783 ASAP2_HUMAN (1-1006): 0: 661: 100.00		NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24
+	Hs
		195787 ASAP2_HUMAN (1-1006): 0: 661: 100.00		P85A_HUMAN (166-379): 4.05e-24: 106: 16.36
+	Hs
		195776 ASAP2_HUMAN (1-1006): 0: 661: 100.00		FYN_HUMAN (7-137): 2.1e-23: 103: 20.30
+	Hs
		195795 ASAP2_HUMAN (1-1006): 0: 661: 100.00		ITSN1_HUMAN (6-70): 3.77e-19: 91.7: 29.23
+	Hs
		195791 ASAP2_HUMAN (1-1006): 0: 661: 100.00		GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81
+	Hs
		195796 ASAP2_HUMAN (1-1006): 0: 661: 100.00		Q5JPT6_HUMAN (8-109): 4.25e-16: 82: 16.35
+	Hs
		195804 ASAP2_HUMAN (1-1006): 0: 661: 100.00		SH3K1_HUMAN (8-109): 4.25e-16: 82: 16.35
+	Hs
		195782 ASAP2_HUMAN (1-1006): 0: 661: 100.00		SRC_HUMAN (203-361): 5.72e-16: 81.3: 31.55
+	Hs
		195777 ASAP2_HUMAN (1-1006): 0: 661: 100.00		YES_HUMAN (6-120): 7.99e-16: 80.8: 23.08
+	Hs
		557696 ACAP1_HUMAN (23-678): 3.93e-128: 407: 19.97		NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24
+	Hs
		924582 ACAP1_HUMAN (23-678): 3.93e-128: 407: 19.97		GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81
+	Hs
		522992 ACAP1_HUMAN (23-678): 3.93e-128: 407: 19.97		SRC_HUMAN (203-361): 5.72e-16: 81.3: 31.55
+	Hs
		523208 AGAP1_HUMAN (136-646): 6.55e-94: 319: 17.69		SRC_HUMAN (203-361): 5.72e-16: 81.3: 31.55
+	Hs
		209145 ARAP1_HUMAN (362-559): 1.21e-18: 94: 31.03		NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00
+	Hs
		655450 AGAP2_HUMAN (136-646): 1.27e-80: 287: 18.58		P85A_HUMAN (166-379): 4.05e-24: 106: 16.36
+	Mm
		403434 AGAP2_MOUSE (136-646): 5.93e-79: 282: 18.39		SRC_MOUSE (6-153): 5.44e-20: 93.3: 17.33
+	Hs
		655498 DP13A_HUMAN (24-438): 1.35e-70: 251: 16.51		P85A_HUMAN (166-379): 4.05e-24: 106: 16.36
+	Hs
		129347 DP13A_HUMAN (24-438): 1.35e-70: 251: 16.51		P85B_HUMAN (21-380): 7.78e-23: 102: 13.10
+	Hs
		412284 ARAP2_HUMAN (120-625): 1.74e-59: 225: 19.77		FYN_HUMAN (7-137): 2.1e-23: 103: 20.30
+	Hs
		411807 ARAP2_HUMAN (120-625): 1.74e-59: 225: 19.77		LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53