HitPredict

Interactor 1: SPY2 (O43597)
Protein sprouty homolog 2 (Spry-2)
Gene: SPRY2

Interactor 2: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.54	0.60	0.573

Property	Details
Experiments	25416956
Identification Methods	two hybrid, two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol GO:0008285 - negative regulation of cell population proliferation
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 315 Hs			1 380
+	Hs
		208142 SPY2_HUMAN (1-315): 1.24e-136: 391: 100.00		ITSN1_HUMAN (6-70): 3.77e-19: 91.7: 29.23
+	Hs
		924668 Q7Z783_HUMAN (1-312): 9.45e-138: 395: 98.72		GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81
+	Hs
		1192446 Q7Z783_HUMAN (1-312): 9.45e-138: 395: 98.72		SH3K1_HUMAN (8-109): 4.25e-16: 82: 16.35
+	Hs
		208125 SPY2_HUMAN (1-315): 1.24e-136: 391: 100.00		GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81
+	Hs
		208145 SPY2_HUMAN (1-315): 1.24e-136: 391: 100.00		Q5JPT6_HUMAN (8-109): 4.25e-16: 82: 16.35
+	Hs
		208177 SPY2_HUMAN (1-315): 1.24e-136: 391: 100.00		SH3K1_HUMAN (8-109): 4.25e-16: 82: 16.35
+	Mm
		1351605 SPY2_MOUSE (1-315): 1.04e-129: 373: 96.20		ITSN1_MOUSE (6-70): 1.15e-18: 90.1: 29.23
+	Mm
		1141168 SPY2_MOUSE (1-315): 1.04e-129: 373: 96.20		GRB2_MOUSE (1-61): 1.04e-18: 85.9: 27.42
+	Mm
		1246943 SPY2_MOUSE (1-315): 1.04e-129: 373: 96.20		SH3K1_MOUSE (8-109): 3.82e-16: 82: 17.31
+	Hs
		208348 SPY1_HUMAN (1-315): 1.06e-110: 325: 51.67		GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81
+	Hs
		208390 SPY1_HUMAN (1-315): 1.06e-110: 325: 51.67		SH3K1_HUMAN (8-109): 4.25e-16: 82: 16.35
+	Mm
		1141172 SPY4_MOUSE (3-305): 1.03e-91: 276: 41.61		GRB2_MOUSE (1-61): 1.04e-18: 85.9: 27.42
+	Hs
		924846 SPY4_HUMAN (4-305): 6.69e-92: 277: 40.78		GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81
+	Mm
		1141169 SPY1_MOUSE (1-315): 8.67e-91: 274: 50.62		GRB2_MOUSE (1-61): 1.04e-18: 85.9: 27.42
+	Mm
		1113312 SPRY3_MOUSE (39-313): 1.21e-88: 268: 43.86		GRB2_MOUSE (1-61): 1.04e-18: 85.9: 27.42
+	Hs
		208416 SPY3_HUMAN (39-305): 3.2e-89: 269: 44.89		GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81
+	Dm
		219699 SPY_DROME (152-294): 4.84e-48: 171: 45.03		GRAP_DROME (204-368): 9.19e-17: 80.1: 30.72
+	Hs
		412242 SPRE1_HUMAN (142-277): 3.35e-39: 145: 25.18		FYN_HUMAN (7-137): 2.1e-23: 103: 20.30
+	Hs
		129314 SPRE1_HUMAN (142-277): 3.35e-39: 145: 25.18		P85B_HUMAN (21-380): 7.78e-23: 102: 13.10