HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: SF3B4 (Q15427)
Splicing factor 3B subunit 4 (Pre-mRNA-splicing factor SF3b 49 kDa subunit) (Spliceosome-associated protein 49) (SAP 49)
Gene: SF3B4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.82	0.50	0.644

Property	Details
Experiments	25416956, 16189514, 32296183, unassigned1304
Identification Methods	two hybrid, validated two hybrid, two hybrid prey pooling approach, two hybrid array, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	-
Homologous interactions	18

Homologous interactions

Color coding for hit scores

	1 380 Hs			1 424
+	Hs
		557702 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		SF3B4_HUMAN (1-424): 5.15e-61: 205: 100.00
+	Hs
		412206 FYN_HUMAN (7-137): 2.1e-23: 103: 20.30		SF3B4_HUMAN (1-424): 5.15e-61: 205: 100.00
+	Ce
		998580 Q95PW9_CAEEL (1-379): 5.2e-75: 239: 37.88		SF3B4_CAEEL (88-364): 3.32e-10: 63.3: 14.79
+	Hs
		1073578 ITSN1_HUMAN (6-70): 3.77e-19: 91.7: 29.23		SF3B4_HUMAN (1-424): 5.15e-61: 205: 100.00
+	Hs
		789117 FRK_HUMAN (3-132): 2.88e-18: 88.3: 18.71		SF3B4_HUMAN (1-424): 5.15e-61: 205: 100.00
+	Hs
		924591 GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81		SF3B4_HUMAN (1-424): 5.15e-61: 205: 100.00
+	Hs
		924560 GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81		HNRPD_HUMAN (3-273): 4.01e-55: 188: 19.12
+	Hs
		1031420 SH3K1_HUMAN (8-109): 4.25e-16: 82: 16.35		PABP4_HUMAN (4-188): 5.81e-40: 153: 38.17
+	Hs
		522960 SRC_HUMAN (203-361): 5.72e-16: 81.3: 31.55		PABP4_HUMAN (4-188): 5.81e-40: 153: 38.17
+	Hs
		433366 YES_HUMAN (6-120): 7.99e-16: 80.8: 23.08		ELAV1_HUMAN (3-183): 2.25e-41: 151: 25.00
+	Ce
		220214 SRC2_CAEEL (1-202): 6.89e-21: 96: 16.75		SF3B4_CAEEL (88-364): 3.32e-10: 63.3: 14.79
+	Hs
		516450 GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81		PABP1_HUMAN (90-181): 1.18e-11: 68.7: 27.17
+	Hs
		516629 SH3K1_HUMAN (8-109): 4.25e-16: 82: 16.35		PABP1_HUMAN (90-181): 1.18e-11: 68.7: 27.17
+	Hs
		516342 SRC_HUMAN (203-361): 5.72e-16: 81.3: 31.55		PABP1_HUMAN (90-181): 1.18e-11: 68.7: 27.17
+	Hs
		523147 SRC_HUMAN (203-361): 5.72e-16: 81.3: 31.55		PABP3_HUMAN (90-181): 8.71e-11: 65.7: 27.17
+	Ce
		666211 SEM5_CAEEL (204-364): 4.9e-16: 78.6: 30.68		Q17350_CAEEL (11-183): 5.03e-10: 63.5: 23.96
+	Ce
		998583 Q9U2T9_CAEEL (51-302): 2.69e-14: 76.6: 18.40		SF3B4_CAEEL (88-364): 3.32e-10: 63.3: 14.79
+	Dm
		993224 GRAP_DROME (204-368): 9.19e-17: 80.1: 30.72		Q9W424_DROME (96-385): 5.7e-09: 59.5: 16.72