HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: WASF2 (Q9Y6W5)
Actin-binding protein WASF2 (Protein WAVE-2) (Verprolin homology domain-containing protein 2) (Wiskott-Aldrich syndrome protein family member 2) (WASP family protein member 2)
Gene: WASF2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	0.60	0.667

Property	Details
Experiments	26186194, 28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol GO:0030032 - lamellipodium assembly
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 498
+	Hs
		209188 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		WASF3_HUMAN (1-498): 4.08e-58: 202: 46.92
+	Hs
		557843 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		WASF2_HUMAN (1-498): 3.76e-64: 218: 100.00
+	Hs
		209133 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		WASF1_HUMAN (1-496): 3.22e-43: 162: 43.19
+	Hs
		557755 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		WASF1_HUMAN (1-496): 3.22e-43: 162: 43.19
+	Hs
		203208 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		SYNJ1_HUMAN (36-496): 2.09e-41: 161: 13.30
+	Ce
		712432 Q95PW9_CAEEL (1-379): 5.2e-75: 239: 37.88		SC161_CAEEL (168-440): 5.7e-21: 98.9: 18.05
+	Hs
		1349289 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		WASF2_HUMAN (1-498): 3.76e-64: 218: 100.00
+	Hs
		655509 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		WASF3_HUMAN (1-498): 4.08e-58: 202: 46.92
+	Hs
		655430 P85A_HUMAN (166-379): 4.05e-24: 106: 16.36		WASF1_HUMAN (1-496): 3.22e-43: 162: 43.19
+	Mm
		643108 P85A_MOUSE (166-379): 4.24e-24: 106: 16.36		WASF1_MOUSE (1-496): 3.12e-42: 160: 43.19
+	Hs
		203228 ITSN1_HUMAN (6-70): 3.77e-19: 91.7: 29.23		SYNJ1_HUMAN (36-496): 2.09e-41: 161: 13.30
+	Hs
		203198 LCK_HUMAN (3-145): 6.3e-19: 90.2: 21.53		SYNJ1_HUMAN (36-496): 2.09e-41: 161: 13.30
+	Hs
		924734 GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81		WASF1_HUMAN (1-496): 3.22e-43: 162: 43.19
+	Hs
		203218 GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81		SYNJ1_HUMAN (36-496): 2.09e-41: 161: 13.30
+	Hs
		203230 SH319_HUMAN (1-57): 2.18e-16: 82.8: 19.30		SYNJ1_HUMAN (36-496): 2.09e-41: 161: 13.30
+	Hs
		203241 SH3K1_HUMAN (8-109): 4.25e-16: 82: 16.35		SYNJ1_HUMAN (36-496): 2.09e-41: 161: 13.30
+	Hs
		442866 HCK_HUMAN (2-145): 5.1e-23: 102: 24.83		CBP_HUMAN (38-468): 2.79e-27: 118: 12.79
+	Hs
		782795 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		TENS2_HUMAN (183-440): 5.01e-11: 67.6: 21.46
+	Hs
		195974 GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81		PLXB1_HUMAN (244-415): 1.75e-13: 75.3: 21.35
+	Hs
		924632 GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81		TENS2_HUMAN (183-440): 5.01e-11: 67.6: 21.46