HitPredict

Interactor 1: BUB3 (O43684)
Mitotic checkpoint protein BUB3
Gene: BUB3

Interactor 2: ACTA (P62736)
Actin, aortic smooth muscle (EC 3.6.4.-) (Alpha-actin-2) (Cell growth-inhibiting gene 46 protein) [Cleaved into: Actin, aortic smooth muscle, intermediate form]
Gene: ACTA2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.65	1.00	0.811

Property	Details
Experiments	28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Actin~WD40
Common GO terms	GO:0005829 - cytosol
Homologous interactions	9

Homologous interactions

Color coding for hit scores

	1 328 Hs			1 377
+	Hs
		211196 BUB3_HUMAN (1-328): 4.65e-94: 284: 100.00		ACTBL_HUMAN (4-377): 0: 712: 87.97
+	At
		862534 RAE1_ARATH (3-324): 5.63e-97: 292: 38.32		ACT11_ARATH (1-377): 0: 620: 87.80
+	Hs
		903280 WDR5_HUMAN (209-320): 6.93e-20: 90.4: 16.07		ACTBL_HUMAN (4-377): 0: 712: 87.97
+	Hs
		792947 LIS1_HUMAN (168-319): 3.74e-31: 122: 14.47		ACTB_HUMAN (4-377): 0: 598: 94.12
+	Hs
		792968 LIS1_HUMAN (168-319): 3.74e-31: 122: 14.47		ACTG_HUMAN (4-377): 0: 598: 94.39
+	Hs
		932318 SNR40_HUMAN (1-121): 1.07e-23: 101: 17.42		ACTC_HUMAN (1-377): 0: 598: 98.41
+	Hs
		891660 WDR5_HUMAN (209-320): 6.93e-20: 90.4: 16.07		ACTB_HUMAN (4-377): 0: 598: 94.12
+	Hs
		903110 WDR5_HUMAN (209-320): 6.93e-20: 90.4: 16.07		ACTG_HUMAN (4-377): 0: 598: 94.39
+	Zm
		73734 B6SJ21_MAIZE (91-295): 1.1e-09: 60.8: 15.11		B4F989_MAIZE (1-377): 0: 621: 87.53