HitPredict

Interactor 1: NCK2 (O55033)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: DOK1 (P97465)
Docking protein 1 (Downstream of tyrosine kinase 1) (p62(dok))
Gene: DOK1

Species: Mus musculus

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	1.00	0.691

Property	Details
Experiments	20697350
Identification Methods	affinity chromatography technology, pull down
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	PH~SH3_1, PH~SH3_9
Common GO terms	GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Mm			1 482
+	Mm
		956524 NCK1_MOUSE (1-379): 6.37e-88: 272: 67.19		DOK1_MOUSE (1-482): 0: 545: 100.00
+	Hs
		209149 NCK2_HUMAN (1-380): 1.15e-93: 287: 96.05		DOK1_HUMAN (1-482): 0: 526: 83.44
+	Hs
		557780 NCK1_HUMAN (1-379): 1.12e-87: 271: 66.93		DOK1_HUMAN (1-482): 0: 526: 83.44
+	Mm
		643076 P85A_MOUSE (24-379): 1.32e-31: 128: 12.57		DOK1_MOUSE (1-482): 0: 545: 100.00
+	Hs
		655452 P85A_HUMAN (24-379): 4.46e-31: 126: 12.57		DOK1_HUMAN (1-482): 0: 526: 83.44
+	Hs
		208960 NCK2_HUMAN (1-380): 1.15e-93: 287: 96.05		DOK2_HUMAN (2-425): 5.48e-106: 323: 34.16
+	Mm
		223005 NCK2_MOUSE (1-380): 1.59e-93: 286: 100.00		DOK2_MOUSE (2-425): 3.71e-101: 311: 33.11
+	Hs
		235041 NCK1_HUMAN (1-379): 1.12e-87: 271: 66.93		DOK2_HUMAN (2-425): 5.48e-106: 323: 34.16
+	Mm
		250957 NCK1_MOUSE (1-379): 6.37e-88: 272: 67.19		DOK2_MOUSE (2-425): 3.71e-101: 311: 33.11
+	Hs
		208999 NCK2_HUMAN (1-380): 1.15e-93: 287: 96.05		IRS1_HUMAN (6-360): 6.38e-68: 237: 16.01
+	Hs
		151716 P85A_HUMAN (24-379): 4.46e-31: 126: 12.57		IRS4_HUMAN (6-406): 7.52e-92: 305: 14.29
+	Hs
		129141 P85B_HUMAN (2-379): 2.19e-30: 124: 12.53		IRS4_HUMAN (6-406): 7.52e-92: 305: 14.29
+	Mm
		643075 P85A_MOUSE (24-379): 1.32e-31: 128: 12.57		IRS2_MOUSE (6-370): 2.17e-73: 253: 13.82
+	Rn
		94391 P85A_RAT (24-379): 1.69e-30: 124: 12.57		F1MAL5_RAT (6-370): 1.89e-72: 251: 13.82
+	Mm
		643063 P85A_MOUSE (24-379): 1.32e-31: 128: 12.57		IRS1_MOUSE (6-365): 2.62e-70: 244: 16.27
+	Hs
		655516 P85A_HUMAN (24-379): 4.46e-31: 126: 12.57		IRS2_HUMAN (6-368): 7.77e-71: 246: 12.78
+	Rn
		724634 P85A_RAT (24-379): 1.69e-30: 124: 12.57		IRS1_RAT (6-365): 6.72e-71: 246: 16.27
+	Hs
		655240 P85A_HUMAN (24-379): 4.46e-31: 126: 12.57		IRS1_HUMAN (6-360): 6.38e-68: 237: 16.01
+	Hs
		129219 P85B_HUMAN (2-379): 2.19e-30: 124: 12.53		IRS1_HUMAN (6-360): 6.38e-68: 237: 16.01
+	Rn
		139726 P85A_RAT (24-379): 1.69e-30: 124: 12.57		O08724_RAT (7-314): 1.51e-61: 210: 19.45