HitPredict

Interactor 1: AP2A1 (O95782)
AP-2 complex subunit alpha-1 (100 kDa coated vesicle protein A) (Adaptor protein complex AP-2 subunit alpha-1) (Adaptor-related protein complex 2 subunit alpha-1) (Alpha-adaptin A) (Alpha1-adaptin) (Clathrin assembly protein complex 2 alpha-A large chain) (Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit)
Gene: AP2A1

Interactor 2: EPN4 (Q14677)
Clathrin interactor 1 (Clathrin-interacting protein localized in the trans-Golgi region) (Clint) (Enthoprotin) (Epsin-4) (Epsin-related protein) (EpsinR)
Gene: CLINT1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.74	0.60	0.670

Property	Details
Experiments	28514442, 12429846, 33961781
Identification Methods	affinity chromatography technology, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0006897 - endocytosis GO:0016020 - membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 977 Hs			1 625
+	Hs
		302148 AP2A2_HUMAN (1-977): 0: 832: 76.97		EPN4_HUMAN (1-625): 2.12e-141: 426: 100.00
+	Hs
		321680 AP2A1_HUMAN (1-977): 0: 904: 100.00		EPN1_HUMAN (8-529): 2.46e-67: 231: 23.55
+	Rn
		88757 D3ZUY8_RAT (1-977): 0: 872: 95.30		EPN1_RAT (8-529): 1.01e-68: 235: 24.63
+	Hs
		925867 AP2B1_HUMAN (34-972): 0: 676: 17.11		EPN4_HUMAN (1-625): 2.12e-141: 426: 100.00
+	Hs
		1014011 AP1B1_HUMAN (34-972): 0: 668: 18.15		EPN4_HUMAN (1-625): 2.12e-141: 426: 100.00
+	Rn
		291395 Q66HM2_RAT (1-977): 0: 845: 79.39		EPN1_RAT (8-529): 1.01e-68: 235: 24.63
+	Rn
		291387 AP2A2_RAT (1-977): 0: 841: 79.18		EPN1_RAT (8-529): 1.01e-68: 235: 24.63
+	Hs
		302230 AP2A2_HUMAN (1-977): 0: 832: 76.97		EPN1_HUMAN (8-529): 2.46e-67: 231: 23.55
+	Hs
		203194 AP2A1_HUMAN (1-977): 0: 904: 100.00		SYNJ1_HUMAN (120-617): 7.92e-35: 143: 10.29
+	Dm
		954828 AP2A_DROME (3-977): 0: 784: 66.09		Q7KU90_DROME (6-624): 1.54e-65: 232: 21.07
+	At
		1228257 AP2A1_ARATH (8-977): 0: 727: 36.46		EPN1_ARATH (7-421): 1.05e-66: 229: 21.81
+	Rn
		923862 AP2B1_RAT (34-972): 0: 676: 17.11		EPN2_RAT (8-556): 7.08e-74: 249: 19.17
+	Rn
		291389 AP2B1_RAT (34-972): 0: 676: 17.11		EPN1_RAT (8-529): 1.01e-68: 235: 24.63
+	Rn
		1144064 Q66HM2_RAT (1-977): 0: 845: 79.39		SYNJ1_RAT (314-625): 4.54e-10: 65: 10.35
+	At
		1147101 AP2A1_ARATH (8-977): 0: 727: 36.46		EPN2_ARATH (7-612): 4.91e-48: 182: 17.53
+	Hs
		926065 AP2B1_HUMAN (34-972): 0: 676: 17.11		EPN1_HUMAN (8-529): 2.46e-67: 231: 23.55
+	Sc
		743416 AP2A_YEAST (10-975): 0: 669: 24.70		ENT1_YEAST (8-250): 7.46e-68: 229: 27.98
+	Hs
		307524 AP2B1_HUMAN (34-972): 0: 676: 17.11		EPN2_HUMAN (8-595): 6.55e-52: 191: 20.40
+	Hs
		203219 AP2B1_HUMAN (34-972): 0: 676: 17.11		SYNJ1_HUMAN (120-617): 7.92e-35: 143: 10.29
+	Hs
		203223 AP1B1_HUMAN (34-972): 0: 668: 18.15		SYNJ1_HUMAN (120-617): 7.92e-35: 143: 10.29