HitPredict

Interactor 1: RAF1 (P04049)
RAF proto-oncogene serine/threonine-protein kinase (EC 2.7.11.1) (Proto-oncogene c-RAF) (cRaf) (Raf-1)
Gene: RAF1

Interactor 2: 1433S (P31947)
14-3-3 protein sigma (Epithelial cell marker protein 1) (Stratifin)
Gene: SFN

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.94	1.00	0.973

Property	Details
Experiments	25852190, 26578655, 31980649, 16041367, 15778465, 35044719, 11697890, 16093354, unassigned2173, 36931259
Identification Methods	affinity chromatography technology, fluorescent resonance energy transfer, unspecified method, anti tag coimmunoprecipitation, cross-linking study, fluorescence polarization spectroscopy, tandem affinity purification, biochemical
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	14-3-3~C1_1
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0007165 - signal transduction GO:0043154 - negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 648 Hs			1 248
+	Hs
		373995 RAF1_HUMAN (1-648): 0: 652: 100.00		1433E_HUMAN (2-246): 4.41e-146: 410: 60.41
+	Hs
		374021 RAF1_HUMAN (1-648): 0: 652: 100.00		1433Z_HUMAN (1-243): 5.4e-136: 384: 70.37
+	Mm
		926872 RAF1_MOUSE (1-648): 0: 647: 97.69		1433Z_MOUSE (1-243): 1.34e-136: 385: 69.96
+	Hs
		373714 RAF1_HUMAN (1-648): 0: 652: 100.00		1433T_HUMAN (1-238): 9.98e-108: 312: 71.85
+	Hs
		542828 BRAF_HUMAN (39-647): 1.29e-170: 507: 55.88		1433S_HUMAN (1-248): 3.48e-158: 440: 100.00
+	Hs
		373771 RAF1_HUMAN (1-648): 0: 652: 100.00		1433B_HUMAN (1-239): 3.41e-100: 293: 68.62
+	Hs
		373992 RAF1_HUMAN (1-648): 0: 652: 100.00		1433G_HUMAN (1-242): 1.86e-98: 289: 65.16
+	Mm
		905358 RAF1_MOUSE (1-648): 0: 647: 97.69		1433G_MOUSE (1-242): 1.86e-98: 289: 65.16
+	Hs
		374091 RAF1_HUMAN (1-648): 0: 652: 100.00		1433F_HUMAN (2-242): 2.78e-96: 283: 64.20
+	Hs
		110869 H7C560_HUMAN (39-647): 6.25e-171: 508: 55.88		1433E_HUMAN (2-246): 4.41e-146: 410: 60.41
+	Hs
		542849 BRAF_HUMAN (39-647): 1.29e-170: 507: 55.88		1433E_HUMAN (2-246): 4.41e-146: 410: 60.41
+	Hs
		110870 H7C560_HUMAN (39-647): 6.25e-171: 508: 55.88		1433Z_HUMAN (1-243): 5.4e-136: 384: 70.37
+	Hs
		542857 BRAF_HUMAN (39-647): 1.29e-170: 507: 55.88		1433Z_HUMAN (1-243): 5.4e-136: 384: 70.37
+	Hs
		542820 BRAF_HUMAN (39-647): 1.29e-170: 507: 55.88		1433T_HUMAN (1-238): 9.98e-108: 312: 71.85
+	Gg
		982836 BRAF_CHICK (38-647): 1.98e-169: 504: 56.96		1433Z_CHICK (1-239): 2.54e-102: 298: 71.13
+	Hs
		542827 BRAF_HUMAN (39-647): 1.29e-170: 507: 55.88		1433B_HUMAN (1-239): 3.41e-100: 293: 68.62
+	Hs
		542847 BRAF_HUMAN (39-647): 1.29e-170: 507: 55.88		1433G_HUMAN (1-242): 1.86e-98: 289: 65.16
+	Gg
		982833 BRAF_CHICK (38-647): 1.98e-169: 504: 56.96		1433G_CHICK (1-242): 1.6e-98: 289: 65.16
+	Hs
		542868 BRAF_HUMAN (39-647): 1.29e-170: 507: 55.88		1433F_HUMAN (2-242): 2.78e-96: 283: 64.20
+	Gg
		982837 BRAF_CHICK (38-647): 1.98e-169: 504: 56.96		Q5ZKJ2_CHICK (2-242): 1.74e-96: 284: 64.20