HitPredict

Interactor 1: UBL4A (P11441)
Ubiquitin-like protein 4A (Ubiquitin-like protein GDX)
Gene: UBL4A

Interactor 2: HUWE1 (Q7Z6Z7)
E3 ubiquitin-protein ligase HUWE1 (EC 2.3.2.26) (ARF-binding protein 1) (ARF-BP1) (HECT, UBA and WWE domain-containing protein 1) (HECT-type E3 ubiquitin transferase HUWE1) (Homologous to E6AP carboxyl terminus homologous protein 9) (HectH9) (Large structure of UREB1) (LASU1) (Mcl-1 ubiquitin ligase E3) (Mule) (Upstream regulatory element-binding protein 1) (URE-B1) (URE-binding protein 1)
Gene: HUWE1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.60	1.00	0.776

Property	Details
Experiments	25147182, 19615732
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association

Property	Details
Interacting Pfam domains	HECT~ubiquitin, UBA~ubiquitin, UBM~ubiquitin
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0006511 - ubiquitin-dependent protein catabolic process and 1 others more... GO:0016020 - membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 157 Hs			1 4374
+	Hs
		475080 UBB_HUMAN (1-76): 1.43e-29: 109: 39.47		HUWE1_HUMAN (1-4374): 0: 3240: 100.00
+	Hs
		475984 UBC_HUMAN (1-77): 1.92e-27: 108: 40.26		HUWE1_HUMAN (1-4374): 0: 3240: 100.00
+	Mm
		924239 RS27A_MOUSE (1-123): 2.97e-40: 134: 28.46		HUWE1_MOUSE (1-4374): 0: 3203: 96.37
+	Mm
		476858 UBC_MOUSE (1-102): 1.77e-28: 111: 31.13		HUWE1_MOUSE (1-4374): 0: 3203: 96.37
+	Rn
		1140970 UBC_RAT (1-102): 9.46e-29: 112: 31.13		Q5YB86_RAT (3515-4372): 0: 682: 24.42
+	Hs
		475140 UBB_HUMAN (1-76): 1.43e-29: 109: 39.47		ITCH_HUMAN (3507-4372): 0: 672: 23.10
+	Hs
		476205 UBC_HUMAN (1-77): 1.92e-27: 108: 40.26		ITCH_HUMAN (3507-4372): 0: 672: 23.10
+	Hs
		125347 RS27A_HUMAN (1-123): 2.9e-40: 134: 28.46		WWP2_HUMAN (3506-4372): 0: 624: 24.57
+	Hs
		924156 RS27A_HUMAN (1-123): 2.9e-40: 134: 28.46		WWP1_HUMAN (3507-4372): 0: 613: 22.55
+	Hs
		125112 UBB_HUMAN (1-76): 1.43e-29: 109: 39.47		WWP2_HUMAN (3506-4372): 0: 624: 24.57
+	Hs
		125113 UBC_HUMAN (1-77): 1.92e-27: 108: 40.26		WWP2_HUMAN (3506-4372): 0: 624: 24.57
+	Hs
		476229 UBC_HUMAN (1-77): 1.92e-27: 108: 40.26		NED4L_HUMAN (3542-4372): 0: 618: 27.53
+	Hs
		476350 UBC_HUMAN (1-77): 1.92e-27: 108: 40.26		WWP1_HUMAN (3507-4372): 0: 613: 22.55
+	Hs
		475196 UBB_HUMAN (1-76): 1.43e-29: 109: 39.47		SMUF2_HUMAN (3771-4371): 0: 611: 33.44
+	Sc
		408371 RS31_YEAST (1-122): 3.56e-31: 111: 29.51		RSP5_YEAST (3659-4372): 0: 595: 27.62
+	Hs
		474953 UBB_HUMAN (1-76): 1.43e-29: 109: 39.47		NEDD4_HUMAN (3104-4372): 2.46e-179: 594: 21.46
+	Hs
		475470 UBC_HUMAN (1-77): 1.92e-27: 108: 40.26		NEDD4_HUMAN (3104-4372): 2.46e-179: 594: 21.46
+	Dm
		89879 UBIQP_DROME (1-79): 1.43e-27: 109: 37.98		D5SHR3_DROME (3965-4373): 1.32e-179: 561: 37.14
+	Sc
		408448 RS31_YEAST (1-122): 3.56e-31: 111: 29.51		TOM1_YEAST (3831-4374): 1.99e-157: 554: 43.77
+	Hs
		475963 UBC_HUMAN (1-77): 1.92e-27: 108: 40.26		HECW1_HUMAN (3082-4373): 1.49e-161: 549: 18.31