HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: ACK1 (Q07912)
Activated CDC42 kinase 1 (ACK-1) (EC 2.7.10.2) (EC 2.7.11.1) (Tyrosine kinase non-receptor protein 2)
Gene: TNK2

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.81	1.00	0.902

Property	Details
Experiments	26496610, 33961781, 21309750, 16137687
Identification Methods	affinity chromatography technology, pull down, anti tag coimmunoprecipitation, peptide array
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	PK_Tyr_Ser-Thr~SH2, PK_Tyr_Ser-Thr~SH3_1
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 1038
+	Mm
		222878 NCK1_MOUSE (1-377): 7.48e-99: 300: 98.94		ACK1_MOUSE (1-1038): 0: 793: 92.70
+	Hs
		209039 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ACK1_HUMAN (1-1038): 0: 805: 100.00
+	Mm
		337637 NCK1_MOUSE (1-377): 7.48e-99: 300: 98.94		ABL1_MOUSE (1-955): 1.72e-152: 484: 20.27
+	Hs
		337337 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ABL1_HUMAN (1-955): 4.84e-144: 462: 19.03
+	Hs
		557666 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		FAK1_HUMAN (6-692): 3.3e-131: 425: 21.01
+	Hs
		557701 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ERBB4_HUMAN (84-754): 3.34e-128: 423: 24.17
+	Hs
		557719 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		LMTK1_HUMAN (84-959): 2.68e-126: 419: 21.75
+	Hs
		557630 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ABL2_HUMAN (1-845): 1.9e-126: 415: 19.68
+	Hs
		557644 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		EPHB1_HUMAN (104-486): 2.42e-125: 408: 26.26
+	Hs
		209036 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		FAK1_HUMAN (6-692): 3.3e-131: 425: 21.01
+	Hs
		209056 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ERBB4_HUMAN (84-754): 3.34e-128: 423: 24.17
+	Hs
		411986 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		FYN_HUMAN (1-400): 2.52e-126: 397: 30.18
+	Mm
		763403 NCK1_MOUSE (1-377): 7.48e-99: 300: 98.94		FYN_MOUSE (1-400): 4.06e-126: 396: 30.18
+	Hs
		209001 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ABL2_HUMAN (1-845): 1.9e-126: 415: 19.68
+	Hs
		209018 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		EPHA4_HUMAN (84-486): 5.91e-125: 407: 23.98
+	Hs
		557643 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		EPHA5_HUMAN (109-396): 1.35e-108: 364: 33.33
+	Hs
		337883 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		EGFR_HUMAN (105-387): 2.98e-55: 211: 39.58
+	Hs
		208970 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		EGFR_HUMAN (105-387): 2.98e-55: 211: 39.58
+	Hs
		209000 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		TXK_HUMAN (121-381): 1.27e-50: 189: 40.46
+	Hs
		238346 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		JAK2_HUMAN (102-413): 4.21e-17: 89: 26.97