HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: MINK1 (Q8N4C8)
Misshapen-like kinase 1 (EC 2.7.11.1) (GCK family kinase MiNK) (MAPK/ERK kinase kinase kinase 6) (MEK kinase kinase 6) (MEKKK 6) (Misshapen/NIK-related kinase) (Mitogen-activated protein kinase kinase kinase kinase 6)
Gene: MINK1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.65	0.60	0.626

Property	Details
Experiments	15469942, 33961781
Identification Methods	two hybrid, pull down, affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0030334 - regulation of cell migration
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 1332
+	Hs
		209112 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		MINK1_HUMAN (1-1332): 0: 1277: 100.00
+	Hs
		557820 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		TNIK_HUMAN (1-1332): 0: 1098: 60.83
+	Hs
		209186 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		TNIK_HUMAN (1-1332): 0: 1098: 60.83
+	Hs
		924703 GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81		MINK1_HUMAN (1-1332): 0: 1277: 100.00
+	Hs
		324734 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		M4K4_HUMAN (1-1332): 0: 1026: 57.14
+	Hs
		208968 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		M4K4_HUMAN (1-1332): 0: 1026: 57.14
+	Mm
		956970 NCK1_MOUSE (1-377): 7.48e-99: 300: 98.94		M4K4_MOUSE (1-1332): 0: 993: 56.32
+	Mm
		949279 SRBS2_MOUSE (46-288): 1.38e-24: 107: 18.85		TNIK_MOUSE (1-1332): 0: 1112: 62.60
+	Hs
		324782 GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81		M4K4_HUMAN (1-1332): 0: 1026: 57.14
+	Hs
		557731 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		M4K3_HUMAN (19-441): 4.42e-110: 370: 37.24
+	Dm
		1182596 NCK_DROME (5-374): 5.6e-68: 225: 42.54		Q7KV88_DROME (3-770): 1.46e-122: 410: 42.14
+	Ce
		1095301 Q95PW9_CAEEL (1-376): 2.62e-70: 227: 38.69		MIG15_CAEEL (5-738): 2.65e-112: 381: 38.26
+	Hs
		557840 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		M4K5_HUMAN (5-307): 6.97e-71: 257: 45.60
+	Hs
		789058 ABL2_HUMAN (83-375): 1.75e-33: 134: 23.05		M3K10_HUMAN (21-845): 1.48e-113: 381: 19.26
+	Hs
		655388 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		M3K11_HUMAN (26-779): 1.45e-120: 397: 21.38
+	Dm
		993188 GRAP_DROME (1-61): 1.53e-17: 82.3: 31.15		Q7KV89_DROME (3-759): 6.63e-122: 408: 42.76
+	Hs
		412163 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		STK4_HUMAN (5-469): 5.1e-119: 381: 32.99
+	Hs
		924683 GRB2_HUMAN (3-61): 2.12e-16: 79.1: 28.81		M4K3_HUMAN (19-441): 4.42e-110: 370: 37.24
+	Hs
		412425 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		M4K5_HUMAN (5-307): 6.97e-71: 257: 45.60
+	Hs
		442897 HCK_HUMAN (2-174): 4.07e-20: 93.7: 17.24		M4K5_HUMAN (5-307): 6.97e-71: 257: 45.60