HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: M4K1 (Q92918)
Mitogen-activated protein kinase kinase kinase kinase 1 (EC 2.7.11.1) (Hematopoietic progenitor kinase) (MAPK/ERK kinase kinase kinase 1) (MEK kinase kinase 1) (MEKKK 1)
Gene: MAP4K1

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.88	0.60	0.727

Property	Details
Experiments	9891069, 11279207, 9346925, 32640226, 22974441, 17474147
Identification Methods	pull down, affinity chromatography technology, two hybrid, unspecified method, peptide array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 833
+	Hs
		209135 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		M4K1_HUMAN (1-833): 0: 901: 100.00
+	Hs
		557840 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		M4K5_HUMAN (7-820): 0: 864: 44.15
+	Hs
		557731 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		M4K3_HUMAN (7-820): 0: 863: 38.76
+	Hs
		655434 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		M4K1_HUMAN (1-833): 0: 901: 100.00
+	Hs
		412291 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		M4K1_HUMAN (1-833): 0: 901: 100.00
+	Hs
		412425 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		M4K5_HUMAN (7-820): 0: 864: 44.15
+	Hs
		442897 HCK_HUMAN (2-174): 4.07e-20: 93.7: 17.24		M4K5_HUMAN (7-820): 0: 864: 44.15
+	Ce
		1095301 Q95PW9_CAEEL (1-376): 2.62e-70: 227: 38.69		MIG15_CAEEL (11-415): 1.01e-110: 365: 35.00
+	Hs
		557820 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		TNIK_HUMAN (11-296): 3.35e-64: 236: 42.57
+	Hs
		557740 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		MINK1_HUMAN (11-296): 6.63e-61: 226: 43.58
+	Hs
		209186 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		TNIK_HUMAN (11-296): 3.35e-64: 236: 42.57
+	Hs
		209112 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		MINK1_HUMAN (11-296): 6.63e-61: 226: 43.58
+	Hs
		298951 SRBS2_HUMAN (64-287): 1.79e-23: 104: 18.26		AKT2_HUMAN (1-337): 6.85e-129: 396: 23.19
+	Mm
		643056 P85A_MOUSE (241-376): 1.59e-22: 101: 19.57		AKT1_MOUSE (1-337): 5.84e-129: 396: 23.12
+	Hs
		655233 P85A_HUMAN (241-376): 1.37e-22: 101: 19.57		AKT1_HUMAN (1-337): 6.85e-129: 396: 23.12
+	Hs
		282266 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		CDKL5_HUMAN (12-589): 4.98e-125: 400: 18.62
+	Hs
		412046 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		AKT1_HUMAN (1-337): 6.85e-129: 396: 23.12
+	Dm
		1182596 NCK_DROME (5-374): 5.6e-68: 225: 42.54		Q7KV88_DROME (11-340): 1.96e-68: 248: 41.31
+	Hs
		412163 FYN_HUMAN (7-173): 7.47e-21: 96: 15.39		STK4_HUMAN (4-437): 2.54e-121: 376: 33.10
+	Mm
		949279 SRBS2_MOUSE (46-288): 1.38e-24: 107: 18.85		TNIK_MOUSE (11-296): 3.3e-64: 236: 42.57