HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: ABI2 (Q9NYB9)
Abl interactor 2 (Abelson interactor 2) (Abi-2) (Abl-binding protein 3) (AblBP3) (Arg-binding protein 1) (ArgBP1)
Gene: ABI2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.62	1.00	0.793

Property	Details
Experiments	26871637, 33961781
Identification Methods	two hybrid, affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	SH2~SH3_1, SH3_1~SH3_1
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0008093 - cytoskeletal anchor activity GO:0016477 - cell migration and 1 others more... GO:0035591 - signaling adaptor activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 513
+	Hs
		557737 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ABI1_HUMAN (1-513): 3.84e-97: 304: 61.00
+	Mm
		223016 NCK2_MOUSE (1-376): 3.75e-90: 277: 66.93		ABI1_MOUSE (1-513): 2.82e-100: 312: 60.04
+	Hs
		209108 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ABI1_HUMAN (1-513): 3.84e-97: 304: 61.00
+	Hs
		209180 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ABI2_HUMAN (1-513): 1.82e-91: 290: 100.00
+	Hs
		557823 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ASAP1_HUMAN (65-510): 8.77e-43: 165: 14.66
+	Hs
		209187 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ASAP1_HUMAN (65-510): 8.77e-43: 165: 14.66
+	Hs
		203208 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		SYNJ1_HUMAN (38-480): 2.57e-33: 137: 12.92
+	Rn
		71363 B2RZ33_RAT (1-377): 8.61e-99: 300: 99.20		SRC8_RAT (228-507): 5.28e-32: 130: 16.07
+	Hs
		557698 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ARHG6_HUMAN (408-508): 2.64e-22: 102: 29.81
+	Hs
		557687 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		ARHG7_HUMAN (451-508): 2.16e-18: 90.8: 41.38
+	Hs
		557655 NCK1_HUMAN (1-377): 1.69e-98: 299: 100.00		GRB2_HUMAN (454-510): 7.13e-18: 84.5: 37.93
+	Hs
		209055 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ARHG6_HUMAN (408-508): 2.64e-22: 102: 29.81
+	Hs
		209051 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ARHG7_HUMAN (451-508): 2.16e-18: 90.8: 41.38
+	Hs
		208967 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		SRBS2_HUMAN (373-509): 1.11e-16: 85.5: 19.87
+	Hs
		209184 NCK2_HUMAN (1-376): 7.1e-90: 277: 68.24		ABI3_HUMAN (262-507): 4.36e-17: 84.9: 23.17
+	Hs
		789012 TEC_HUMAN (27-377): 4.7e-38: 146: 17.63		ASAP1_HUMAN (65-510): 8.77e-43: 165: 14.66
+	Hs
		548654 TEC_HUMAN (27-377): 4.7e-38: 146: 17.63		VAV_HUMAN (276-513): 8.6e-32: 131: 17.86
+	Hs
		788982 TEC_HUMAN (27-377): 4.7e-38: 146: 17.63		GRB2_HUMAN (454-510): 7.13e-18: 84.5: 37.93
+	Hs
		298959 ABL2_HUMAN (83-375): 1.75e-33: 134: 23.05		SRBS2_HUMAN (373-509): 1.11e-16: 85.5: 19.87
+	Hs
		789054 ABL2_HUMAN (83-375): 1.75e-33: 134: 23.05		GRB2_HUMAN (454-510): 7.13e-18: 84.5: 37.93