HitPredict

Interactor 1: P85A (P27986)
Phosphatidylinositol 3-kinase regulatory subunit alpha (PI3-kinase regulatory subunit alpha) (PI3K regulatory subunit alpha) (PtdIns-3-kinase regulatory subunit alpha) (Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha) (PI3-kinase subunit p85-alpha) (PtdIns-3-kinase regulatory subunit p85-alpha)
Gene: PIK3R1

Interactor 2: CRK (P46108)
Adapter molecule crk (Proto-oncogene c-Crk) (p38)
Gene: CRK

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.88	1.00	0.939

Property	Details
Experiments	11418612, 25814554, 12878163, 10962563, 19380743, unassigned1126
Identification Methods	affinity chromatography technology, two hybrid, validated two hybrid, two hybrid array, tandem affinity purification
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	PI3K_P85_iSH2~SH2, SH2~SH3_1, SH2~SH3_2
Common GO terms	GO:0001784 - phosphotyrosine residue binding GO:0005159 - insulin-like growth factor receptor binding GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol and 2 others more... GO:0005886 - plasma membrane GO:0016020 - membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 724 Hs			1 304
+	Mm
		643099 P85A_MOUSE (1-724): 0: 592: 95.99		CRK_MOUSE (1-304): 5.53e-68: 215: 99.67
+	Hs
		655266 P85A_HUMAN (1-724): 0: 596: 100.00		CRKL_HUMAN (2-302): 4.5e-57: 187: 48.07
+	Mm
		643068 P85A_MOUSE (1-724): 0: 592: 95.99		CRKL_MOUSE (2-302): 6.92e-57: 187: 47.10
+	Hs
		548645 P85A_HUMAN (1-724): 0: 596: 100.00		VAV_HUMAN (11-189): 6.6e-47: 170: 28.21
+	Hs
		655500 P85A_HUMAN (1-724): 0: 596: 100.00		VAV3_HUMAN (11-189): 1.56e-33: 131: 26.15
+	Hs
		580936 P85A_HUMAN (1-724): 0: 596: 100.00		PLCG1_HUMAN (1-302): 7.99e-27: 112: 15.39
+	Hs
		562627 P85A_HUMAN (1-724): 0: 596: 100.00		PLCG2_HUMAN (1-301): 1.03e-25: 109: 15.89
+	Hs
		129237 P85B_HUMAN (4-719): 6.38e-162: 486: 57.24		CRK_HUMAN (1-304): 7.82e-68: 215: 100.00
+	Hs
		655306 P85A_HUMAN (1-724): 0: 596: 100.00		GRB2_HUMAN (135-297): 2.78e-25: 102: 15.67
+	Mm
		643091 P85A_MOUSE (1-724): 0: 592: 95.99		GRB2_MOUSE (135-297): 1.43e-25: 103: 15.67
+	Hs
		129238 P85B_HUMAN (4-719): 6.38e-162: 486: 57.24		CRKL_HUMAN (2-302): 4.5e-57: 187: 48.07
+	Hs
		557615 P85A_HUMAN (1-724): 0: 596: 100.00		NCK1_HUMAN (2-100): 1.55e-10: 63.3: 18.00
+	Hs
		655386 P85A_HUMAN (1-724): 0: 596: 100.00		ITSN1_HUMAN (150-302): 3.02e-10: 63.3: 13.87
+	Hs
		655439 P85A_HUMAN (1-724): 0: 596: 100.00		SH3K1_HUMAN (114-192): 4.08e-10: 62.5: 22.22
+	Hs
		129269 P85B_HUMAN (4-719): 6.38e-162: 486: 57.24		GRB2_HUMAN (135-297): 2.78e-25: 102: 15.67
+	Hs
		802683 P55G_HUMAN (271-723): 1.4e-104: 329: 72.31		CRK_HUMAN (1-304): 7.82e-68: 215: 100.00
+	Hs
		924735 P55G_HUMAN (271-723): 1.4e-104: 329: 72.31		GRB2_HUMAN (135-297): 2.78e-25: 102: 15.67
+	Hs
		557757 P55G_HUMAN (271-723): 1.4e-104: 329: 72.31		NCK1_HUMAN (2-100): 1.55e-10: 63.3: 18.00
+	Hs
		64627 RHG32_HUMAN (14-613): 1.13e-61: 227: 13.99		NCK2_HUMAN (2-100): 3.06e-11: 65.2: 18.00
+	Hs
		64651 RHG32_HUMAN (14-613): 1.13e-61: 227: 13.99		NCK1_HUMAN (2-100): 1.55e-10: 63.3: 18.00