HitPredict

Interactor 1: ALY1 (P36117)
Arrestin-related trafficking adapter 6 (Arrestin-like protein 1)
Gene: ALY1

Interactor 2: RSP5 (P39940)
E3 ubiquitin-protein ligase RSP5 (EC 2.3.2.26) (HECT-type E3 ubiquitin transferase RSP5) (Reverses SPT-phenotype protein 5)
Gene: RSP5

Species: Saccharomyces cerevisiae S288C

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.88	0.60	0.730

Property	Details
Experiments	26508657, 17551511, 18976803, 20489023, 23824189, 16429126
Identification Methods	affinity chromatography technology, enzymatic study, pull down, anti tag coimmunoprecipitation, tandem affinity purification
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005886 - plasma membrane GO:0070086 - ubiquitin-dependent endocytosis GO:2000397 - positive regulation of ubiquitin-dependent endocytosis
Homologous interactions	6

Homologous interactions

Color coding for hit scores

	1 915 Sc			1 809
+	Sc
		765309 ALY2_YEAST (12-904): 0: 868: 39.14		RSP5_YEAST (1-809): 0: 825: 100.00
+	Sc
		765337 ART5_YEAST (59-498): 4.33e-119: 376: 14.64		RSP5_YEAST (1-809): 0: 825: 100.00
+	Sc
		765366 ROD1_YEAST (59-494): 2.32e-79: 276: 18.53		RSP5_YEAST (1-809): 0: 825: 100.00
+	Sc
		765302 ROG3_YEAST (271-494): 1.95e-60: 221: 20.71		RSP5_YEAST (1-809): 0: 825: 100.00
+	Sp
		1264987 ALY1_SCHPO (30-132): 9.72e-39: 153: 36.89		PUB1_SCHPO (4-809): 0: 782: 63.82
+	Sp
		145423 ALY1_SCHPO (30-132): 9.72e-39: 153: 36.89		PUB3_SCHPO (4-809): 0: 733: 54.05