HitPredict

Interactor 1: GBRL2 (P60520)
Gamma-aminobutyric acid receptor-associated protein-like 2 (GABA(A) receptor-associated protein-like 2) (Ganglioside expression factor 2) (GEF-2) (General protein transport factor p16) (Golgi-associated ATPase enhancer of 16 kDa) (GATE-16) (MAP1 light chain 3-related protein)
Gene: GABARAPL2

Interactor 2: UBA5 (Q9GZZ9)
Ubiquitin-like modifier-activating enzyme 5 (Ubiquitin-activating enzyme 5) (ThiFP1) (UFM1-activating enzyme) (Ubiquitin-activating enzyme E1 domain-containing protein 1)
Gene: UBA5

Species: Homo sapiens

Source: mint

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.95	1.00	0.979

Property	Details
Experiments	28514442, 33961781, 20562859, 25416956, 16189514, 32296183, 21900206, unassigned1304
Identification Methods	affinity chromatography technology, two hybrid, anti tag coimmunoprecipitation, two hybrid prey pooling approach, two hybrid array, validated two hybrid, pull down, two hybrid pooling approach
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	ATG8~ThiF
Common GO terms	GO:0005737 - cytoplasm GO:0005789 - endoplasmic reticulum membrane GO:0005794 - Golgi apparatus GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 117 Hs			1 404
+	Hs
		306308 GBRAP_HUMAN (1-117): 7.55e-57: 173: 57.27		UBA5_HUMAN (1-404): 1.8e-151: 436: 100.00
+	Hs
		1326964 GBRL1_HUMAN (1-116): 7.89e-56: 170: 61.21		UBA5_HUMAN (1-404): 1.8e-151: 436: 100.00
+	Hs
		1315800 MLP3C_HUMAN (5-116): 5.09e-45: 144: 42.11		UBA5_HUMAN (1-404): 1.8e-151: 436: 100.00
+	Hs
		1326135 MLP3B_HUMAN (2-117): 6.34e-44: 141: 38.14		UBA5_HUMAN (1-404): 1.8e-151: 436: 100.00
+	Sc
		745982 ATG8_YEAST (1-116): 1.06e-58: 178: 56.03		ATG7_YEAST (39-351): 1.12e-80: 261: 15.76
+	At
		1277811 ATG8D_ARATH (3-117): 3.37e-62: 186: 57.39		ATG7_ARATH (24-350): 2.98e-60: 208: 13.94
+	At
		1258019 ATG8F_ARATH (2-117): 1.18e-61: 185: 62.07		ATG7_ARATH (24-350): 2.98e-60: 208: 13.94
+	Dm
		1177993 Q9W2S2_DROME (1-117): 5.35e-58: 176: 57.27		Q7JY94_DROME (39-320): 1.61e-61: 212: 15.74
+	Dm
		55857 Q9W2S2_DROME (1-117): 5.35e-58: 176: 57.27		A1ZBA9_DROME (39-320): 6.28e-63: 212: 15.74
+	Hs
		311683 GBRL2_HUMAN (1-117): 1.08e-58: 178: 100.00		ATG7_HUMAN (19-351): 3.67e-60: 208: 13.69
+	Mm
		891376 GBRL2_MOUSE (1-117): 1.08e-58: 178: 100.00		ATG7_MOUSE (19-351): 2.5e-59: 206: 13.97
+	Hs
		305873 GBRAP_HUMAN (1-117): 7.55e-57: 173: 57.27		ATG7_HUMAN (19-351): 3.67e-60: 208: 13.69
+	Mm
		1322119 GBRAP_MOUSE (1-117): 7.55e-57: 173: 57.27		ATG7_MOUSE (19-351): 2.5e-59: 206: 13.97
+	Hs
		311756 GBRL1_HUMAN (1-116): 7.89e-56: 170: 61.21		ATG7_HUMAN (19-351): 3.67e-60: 208: 13.69
+	Ce
		104029 LGG1_CAEEL (1-116): 2.15e-55: 170: 57.76		G5EBK4_CAEEL (43-279): 1.67e-53: 190: 15.47
+	Hs
		311750 MLP3C_HUMAN (5-116): 5.09e-45: 144: 42.11		ATG7_HUMAN (19-351): 3.67e-60: 208: 13.69
+	Hs
		311758 MLP3A_HUMAN (5-117): 2.01e-45: 144: 41.74		ATG7_HUMAN (19-351): 3.67e-60: 208: 13.69
+	Mm
		1265088 MLP3A_MOUSE (5-117): 2.01e-45: 144: 41.74		ATG7_MOUSE (19-351): 2.5e-59: 206: 13.97
+	Hs
		311755 MLP3B_HUMAN (2-117): 6.34e-44: 141: 38.14		ATG7_HUMAN (19-351): 3.67e-60: 208: 13.69
+	Mm
		1321768 MLP3B_MOUSE (2-116): 2.53e-44: 141: 38.46		ATG7_MOUSE (19-351): 2.5e-59: 206: 13.97