HitPredict

Interactor 1: 1433G (P61981)
14-3-3 protein gamma (Protein kinase C inhibitor protein 1) (KCIP-1) [Cleaved into: 14-3-3 protein gamma, N-terminally processed]
Gene: YWHAG

Interactor 2: M3K3 (Q99759)
Mitogen-activated protein kinase kinase kinase 3 (EC 2.7.11.25) (MAPK/ERK kinase kinase 3) (MEK kinase 3) (MEKK 3)
Gene: MAP3K3

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.89	0.60	0.734

Property	Details
Experiments	33961781, 36931259, 14743216, 32707033, 36736316, 28514442
Identification Methods	affinity chromatography technology, pull down, tandem affinity purification, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 247 Hs			1 626
+	Hs
		909957 1433E_HUMAN (1-244): 2.02e-141: 398: 63.53		M3K3_HUMAN (1-626): 0: 606: 100.00
+	Hs
		910191 1433E_HUMAN (1-244): 2.02e-141: 398: 63.53		M3K2_HUMAN (1-622): 3.22e-174: 510: 64.33
+	Mm
		910275 1433E_MOUSE (1-244): 2.02e-141: 398: 63.53		DCLK2_MOUSE (21-624): 1.04e-138: 424: 16.51
+	Hs
		909811 1433E_HUMAN (1-244): 2.02e-141: 398: 63.53		DCLK2_HUMAN (21-624): 3.27e-138: 422: 16.19
+	Hs
		166549 1433E_HUMAN (1-244): 2.02e-141: 398: 63.53		DCLK1_HUMAN (21-624): 5.2e-136: 416: 16.90
+	Hs
		904475 1433G_HUMAN (1-247): 3.15e-139: 392: 100.00		KPCD_HUMAN (22-625): 8.67e-139: 421: 17.86
+	Hs
		166546 1433G_HUMAN (1-247): 3.15e-139: 392: 100.00		DCLK1_HUMAN (21-624): 5.2e-136: 416: 16.90
+	Mm
		910345 1433E_MOUSE (1-244): 2.02e-141: 398: 63.53		DCLK1_MOUSE (21-624): 2.8e-133: 409: 16.54
+	Hs
		904468 1433G_HUMAN (1-247): 3.15e-139: 392: 100.00		KPCT_HUMAN (40-625): 2e-133: 408: 15.76
+	Zm
		45993 B6SRI4_MAIZE (2-245): 2.63e-140: 395: 60.16		A0A1Q0ZG94_MAIZE (20-625): 5.87e-126: 403: 17.13
+	At
		1280891 14337_ARATH (3-244): 3.18e-142: 400: 60.25		CIPKC_ARATH (342-626): 1.65e-129: 391: 25.78
+	At
		788672 14333_ARATH (1-244): 4.4e-142: 400: 61.79		CIPKC_ARATH (342-626): 1.65e-129: 391: 25.78
+	At
		965360 14332_ARATH (4-244): 1.39e-141: 398: 61.32		CIPKC_ARATH (342-626): 1.65e-129: 391: 25.78
+	Hs
		910114 1433E_HUMAN (1-244): 2.02e-141: 398: 63.53		PAK5_HUMAN (37-624): 7.39e-124: 384: 21.13
+	Hs
		681770 1433E_HUMAN (1-244): 2.02e-141: 398: 63.53		AKT1_HUMAN (247-622): 1.65e-126: 383: 21.67
+	Hs
		681766 1433G_HUMAN (1-247): 3.15e-139: 392: 100.00		AKT1_HUMAN (247-622): 1.65e-126: 383: 21.67
+	Hs
		904516 1433G_HUMAN (1-247): 3.15e-139: 392: 100.00		PAK2_HUMAN (123-624): 8.76e-126: 383: 23.91
+	At
		788620 14333_ARATH (1-244): 4.4e-142: 400: 61.79		M3K2_ARATH (343-622): 6.39e-115: 359: 46.67
+	Hs
		909513 1433E_HUMAN (1-244): 2.02e-141: 398: 63.53		M3K1_HUMAN (362-619): 9.22e-53: 197: 44.03
+	Hs
		904518 1433G_HUMAN (1-247): 3.15e-139: 392: 100.00		M3K1_HUMAN (362-619): 9.22e-53: 197: 44.03