HitPredict

Interactor 1: RBX1 (P62877)
E3 ubiquitin-protein ligase RBX1 (EC 2.3.2.27) (EC 2.3.2.32) (E3 ubiquitin-protein transferase RBX1) (Protein ZYP) (RING finger protein 75) (RING-box protein 1) (Rbx1) (Regulator of cullins 1) (ROC1) [Cleaved into: E3 ubiquitin-protein ligase RBX1, N-terminally processed (E3 ubiquitin-protein transferase RBX1, N-terminally processed)]
Gene: RBX1

Interactor 2: CUL5 (Q93034)
Cullin-5 (CUL-5) (Vasopressin-activated calcium-mobilizing receptor 1) (VACM-1)
Gene: CUL5

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score Annotation score Interaction score
0.94 1.00 0.973

The method score is based on:

Property Details
Experiments 19250909, 25912140, 30349055, 10230407, 35551201, 22405651, 31253590, 18805092, 11861641, 15574593
Identification Methods enzymatic study, affinity chromatography technology, two hybrid, pull down, x-ray crystallography
Interaction Type direct interaction, physical association, association

The annotation score is based on:

Property Details
Interacting Pfam domains Cullin~zf-rbx1, Cullin_Nedd8~zf-rbx1
Common GO terms GO:0005634 - nucleus
GO:0005829 - cytosol
GO:0016567 - protein ubiquitination
GO:0019005 - SCF ubiquitin ligase complex
GO:0031146 - SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
and 2 others

more...
Homologous interactions 20

Homologous interactions (Top 20)

Color coding for hit scores

1 108
Hs

1 780
+ Hs
+ Hs
+ Mm
+ Mm
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+ At
+ Mm
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+ Dm
+ At
+ Hs
+ Dm
+ Dm
+ At
+ Ce
+ Sp
+ Ce
+ Dm
+ Sp

Service provided by Combinatics, Japan

Last updated: 15 Jul 2024