HitPredict

Interactor 1: UBC9 (P63279)
SUMO-conjugating enzyme UBC9 (EC 2.3.2.-) (RING-type E3 SUMO transferase UBC9) (SUMO-protein ligase) (Ubiquitin carrier protein 9) (Ubiquitin carrier protein I) (Ubiquitin-conjugating enzyme E2 I) (Ubiquitin-protein ligase I) (p18)
Gene: UBE2I

Interactor 2: PIAS3 (Q9Y6X2)
E3 SUMO-protein ligase PIAS3 (EC 2.3.2.-) (E3 SUMO-protein transferase PIAS3) (Protein inhibitor of activated STAT protein 3)
Gene: PIAS3

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.62	1.00	0.791

Property	Details
Experiments	20936779, 21988832
Identification Methods	two hybrid, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	PINIT~UQ_con, UQ_con~zf-MIZ
Common GO terms	GO:0000122 - negative regulation of transcription by RNA polymerase II GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0016925 - protein sumoylation GO:0019789 - SUMO transferase activity
Homologous interactions	14

Homologous interactions

Color coding for hit scores

	1 158 Hs			1 628
+	Mm
		222292 UBC9_MOUSE (1-158): 1.43e-70: 211: 100.00		PIAS3_MOUSE (1-628): 0: 867: 96.98
+	Hs
		278043 UBC9_HUMAN (1-158): 1.43e-70: 211: 100.00		PIAS1_HUMAN (1-628): 0: 728: 56.69
+	Hs
		278297 Q7KZS0_HUMAN (1-137): 2.27e-71: 214: 100.00		PIAS2_HUMAN (1-550): 0: 651: 59.00
+	Hs
		278258 UBC9_HUMAN (1-158): 1.43e-70: 211: 100.00		PIAS2_HUMAN (1-550): 0: 651: 59.00
+	Hs
		930294 UBC9_HUMAN (1-158): 1.43e-70: 211: 100.00		PIAS4_HUMAN (2-477): 2.11e-177: 514: 46.71
+	Dm
		1124421 Q7KNM2_DROME (1-158): 1.41e-66: 201: 85.44		Q5BIG7_DROME (4-519): 1.69e-163: 480: 42.24
+	Dm
		52662 Q7KNM2_DROME (1-158): 1.41e-66: 201: 85.44		A1Z7P7_DROME (6-519): 4.54e-162: 477: 42.21
+	Dm
		1181479 Q7KNM2_DROME (1-158): 1.41e-66: 201: 85.44		Q9XYM5_DROME (10-519): 2.07e-161: 474: 42.34
+	At
		1114921 SCE1_ARATH (2-155): 5.4e-72: 215: 66.23		SIZ1_ARATH (5-623): 1.75e-120: 380: 21.29
+	Ce
		1276138 UBC9_CAEEL (1-157): 3.2e-68: 205: 76.43		GEI17_CAEEL (130-461): 2.36e-98: 319: 32.75
+	Dm
		1176864 Q7KNM2_DROME (1-158): 1.41e-66: 201: 85.44		Q7JR52_DROME (88-415): 1.01e-60: 221: 22.67
+	Sc
		841451 UBC9_YEAST (1-156): 3.53e-61: 187: 56.41		SIZ2_YEAST (3-506): 2.24e-66: 232: 19.21
+	Sc
		841429 UBC9_YEAST (1-156): 3.53e-61: 187: 56.41		SIZ1_YEAST (3-459): 1.06e-51: 193: 18.72
+	At
		98430 SCE1_ARATH (2-155): 5.4e-72: 215: 66.23		PIAL2_ARATH (208-511): 3.99e-36: 146: 21.97