HitPredict

Interactor 1: SMAD3 (P84022)
Mothers against decapentaplegic homolog 3 (MAD homolog 3) (Mad3) (Mothers against DPP homolog 3) (hMAD-3) (JV15-2) (SMAD family member 3) (SMAD 3) (Smad3) (hSMAD3)
Gene: SMAD3

Interactor 2: WWP1 (Q9H0M0)
NEDD4-like E3 ubiquitin-protein ligase WWP1 (EC 2.3.2.26) (Atrophin-1-interacting protein 5) (AIP5) (HECT-type E3 ubiquitin transferase WWP1) (TGIF-interacting ubiquitin ligase 1) (Tiul1) (WW domain-containing protein 1)
Gene: WWP1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.93	0.60	0.750

Property	Details
Experiments	25416956, 25910212, 15221015, 32296183, 15359284, 15946939, unassigned1304
Identification Methods	two hybrid, affinity chromatography technology, anti tag coimmunoprecipitation, two hybrid prey pooling approach, two hybrid array, validated two hybrid, two hybrid bait and prey pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 425 Hs			1 922
+	Hs
		1078654 SMAD2_HUMAN (1-425): 0: 589: 83.73		WWP1_HUMAN (1-922): 0: 953: 100.00
+	Hs
		950248 SMAD3_HUMAN (1-425): 0: 636: 100.00		ITCH_HUMAN (1-922): 0: 884: 57.14
+	Hs
		1078757 SMAD1_HUMAN (1-425): 0: 559: 60.09		WWP1_HUMAN (1-922): 0: 953: 100.00
+	Hs
		125369 SMAD3_HUMAN (1-425): 0: 636: 100.00		WWP2_HUMAN (1-922): 0: 841: 57.84
+	Hs
		1078624 SMAD2_HUMAN (1-425): 0: 589: 83.73		ITCH_HUMAN (1-922): 0: 884: 57.14
+	Hs
		950249 SMAD3_HUMAN (1-425): 0: 636: 100.00		NED4L_HUMAN (1-919): 0: 822: 33.16
+	Mm
		1143948 SMAD2_MOUSE (1-425): 0: 591: 83.73		ITCH_MOUSE (1-922): 0: 862: 57.54
+	Hs
		125487 SMAD2_HUMAN (1-425): 0: 589: 83.73		WWP2_HUMAN (1-922): 0: 841: 57.84
+	Hs
		1078629 SMAD2_HUMAN (1-425): 0: 589: 83.73		NED4L_HUMAN (1-919): 0: 822: 33.16
+	Dm
		785516 MAD_DROME (1-425): 0: 571: 61.09		SUDX_DROME (14-922): 0: 832: 52.00
+	Hs
		808087 SMAD3_HUMAN (1-425): 0: 636: 100.00		NEDD4_HUMAN (84-919): 0: 723: 33.52
+	Hs
		808134 SMAD2_HUMAN (1-425): 0: 589: 83.73		NEDD4_HUMAN (84-919): 0: 723: 33.52
+	Hs
		808135 SMAD1_HUMAN (1-425): 0: 559: 60.09		NEDD4_HUMAN (84-919): 0: 723: 33.52
+	Hs
		950291 SMAD3_HUMAN (1-425): 0: 636: 100.00		SMUF1_HUMAN (6-451): 5.28e-38: 154: 15.82
+	Hs
		950287 SMAD3_HUMAN (1-425): 0: 636: 100.00		SMUF2_HUMAN (7-381): 2.95e-31: 133: 16.24
+	Hs
		1078671 SMAD2_HUMAN (1-425): 0: 589: 83.73		SMUF1_HUMAN (6-451): 5.28e-38: 154: 15.82
+	Hs
		1078667 SMAD2_HUMAN (1-425): 0: 589: 83.73		SMUF2_HUMAN (7-381): 2.95e-31: 133: 16.24
+	Mm
		56826 SMAD1_MOUSE (1-425): 0: 560: 59.87		SMUF2_MOUSE (7-381): 5.96e-31: 132: 16.24
+	Dm
		785477 MAD_DROME (1-425): 0: 571: 61.09		SMUF1_DROME (16-538): 1.82e-25: 115: 13.02
+	Mm
		936618 SMAD1_MOUSE (1-425): 0: 560: 59.87		SMUF1_MOUSE (276-436): 5.09e-16: 84.9: 21.14