HitPredict

Interactor 1: H33 (P84243)
Histone H3.3
Gene: H3-3A

Interactor 2: ASF1A (Q9Y294)
Histone chaperone ASF1A (Anti-silencing function protein 1 homolog A) (hAsf1) (hAsf1a) (CCG1-interacting factor A) (CIA) (hCIA)
Gene: ASF1A

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.94	1.00	0.972

Property	Details
Experiments	18971269, 27217568, 23828041, 33961781, 36089195, 14718166, 20504901, 23075851, 24209620
Identification Methods	affinity chromatography technology, proximity labelling technology, pull down, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	ASF1_hist_chap~Histone
Common GO terms	GO:0001649 - osteoblast differentiation GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0006334 - nucleosome assembly GO:0032991 - protein-containing complex and 1 others more... GO:0042692 - muscle cell differentiation
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 136 Hs			1 204
+	Hs
		998963 H31_HUMAN (1-136): 3.09e-60: 183: 96.32		ASF1A_HUMAN (1-204): 2.32e-128: 361: 100.00
+	Mm
		999035 H31_MOUSE (1-136): 3.09e-60: 183: 96.32		ASF1A_MOUSE (1-204): 1.46e-128: 361: 99.51
+	Mm
		1017372 H33_MOUSE (1-136): 1.13e-59: 182: 100.00		ASF1A_MOUSE (1-204): 1.46e-128: 361: 99.51
+	Gg
		1017386 H33_CHICK (1-136): 1.13e-59: 182: 100.00		ASF1_CHICK (1-204): 2.32e-128: 361: 100.00
+	Hs
		1162086 H31T_HUMAN (1-136): 2.59e-59: 181: 93.38		ASF1A_HUMAN (1-204): 2.32e-128: 361: 100.00
+	Mm
		1016768 H32_MOUSE (1-136): 5.34e-59: 180: 97.06		ASF1A_MOUSE (1-204): 1.46e-128: 361: 99.51
+	Gg
		1016774 H32_CHICK (1-136): 5.34e-59: 180: 97.06		ASF1_CHICK (1-204): 2.32e-128: 361: 100.00
+	Hs
		1258986 H32_HUMAN (1-136): 5.34e-59: 180: 97.06		ASF1A_HUMAN (1-204): 2.32e-128: 361: 100.00
+	Hs
		998868 H31_HUMAN (1-136): 3.09e-60: 183: 96.32		ASF1B_HUMAN (1-204): 9.3e-121: 342: 70.59
+	Hs
		1017251 H33_HUMAN (1-136): 1.13e-59: 182: 100.00		ASF1B_HUMAN (1-204): 9.3e-121: 342: 70.59
+	Hs
		1162057 H31T_HUMAN (1-136): 2.59e-59: 181: 93.38		ASF1B_HUMAN (1-204): 9.3e-121: 342: 70.59
+	Mm
		999037 H31_MOUSE (1-136): 3.09e-60: 183: 96.32		ASF1B_MOUSE (1-204): 7.97e-117: 332: 72.06
+	Mm
		1017376 H33_MOUSE (1-136): 1.13e-59: 182: 100.00		ASF1B_MOUSE (1-204): 7.97e-117: 332: 72.06
+	Mm
		1016770 H32_MOUSE (1-136): 5.34e-59: 180: 97.06		ASF1B_MOUSE (1-204): 7.97e-117: 332: 72.06
+	Hs
		883710 CENPA_HUMAN (9-133): 2.43e-45: 146: 50.76		ASF1B_HUMAN (1-204): 9.3e-121: 342: 70.59
+	Dm
		87252 H33A_DROME (1-136): 1.13e-59: 182: 100.00		ASF1_DROME (1-203): 1.05e-97: 284: 56.88
+	Dm
		381720 H3_DROME (1-136): 5.34e-59: 180: 97.06		ASF1_DROME (1-203): 1.05e-97: 284: 56.88
+	Sp
		267799 H31_SCHPO (1-136): 1.12e-57: 177: 91.91		ASF1_SCHPO (1-169): 1.83e-84: 252: 51.43
+	Sc
		736700 H3_YEAST (1-136): 3.63e-54: 168: 90.44		ASF1_YEAST (1-168): 7.83e-88: 261: 54.76