HitPredict

Interactor 1: RFWD3 (Q6PCD5)
E3 ubiquitin-protein ligase RFWD3 (EC 2.3.2.27) (RING finger and WD repeat domain-containing protein 3) (RING finger protein 201)
Gene: RFWD3

Interactor 2: UBE2W (Q96B02)
Ubiquitin-conjugating enzyme E2 W (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme W) (N-terminal E2 ubiquitin-conjugating enzyme) (EC 2.3.2.25) (N-terminus-conjugating E2) (Ubiquitin carrier protein W) (Ubiquitin-conjugating enzyme 16) (UBC-16) (Ubiquitin-protein ligase W)
Gene: UBE2W

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.48	1.00	0.698

Property	Details
Experiments	19549727
Identification Methods	two hybrid, two hybrid array
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_2
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0005737 - cytoplasm
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 774 Hs			1 151
+	Hs
		1160856 RFWD3_HUMAN (1-774): 0: 851: 100.00		UB2D4_HUMAN (4-150): 7.3e-70: 208: 35.37
+	Hs
		849343 RFWD3_HUMAN (1-774): 0: 851: 100.00		UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69
+	Hs
		919753 RFWD3_HUMAN (1-774): 0: 851: 100.00		UB2D2_HUMAN (4-150): 1.14e-68: 205: 33.33
+	Hs
		895816 RFWD3_HUMAN (1-774): 0: 851: 100.00		UB2D3_HUMAN (4-150): 3.12e-67: 202: 33.33
+	Hs
		1160836 RFWD3_HUMAN (1-774): 0: 851: 100.00		UB2E3_HUMAN (1-150): 1.13e-65: 200: 35.33
+	Hs
		851824 RFWD3_HUMAN (1-774): 0: 851: 100.00		UB2E1_HUMAN (1-150): 3.64e-64: 196: 34.00
+	Zm
		42874 A0A1D6P951_MAIZE (286-771): 3.73e-133: 408: 22.39		B4FC97_MAIZE (4-150): 5.6e-69: 206: 32.65
+	Hs
		1318719 TRIM5_HUMAN (283-645): 1.97e-56: 202: 13.90		UB2D4_HUMAN (4-150): 7.3e-70: 208: 35.37
+	Hs
		849439 TRIM5_HUMAN (283-645): 1.97e-56: 202: 13.90		UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69
+	Hs
		919853 TRIM5_HUMAN (283-645): 1.97e-56: 202: 13.90		UB2D2_HUMAN (4-150): 1.14e-68: 205: 33.33
+	Hs
		895905 TRIM5_HUMAN (283-645): 1.97e-56: 202: 13.90		UB2D3_HUMAN (4-150): 3.12e-67: 202: 33.33
+	Hs
		1284884 TRIM5_HUMAN (283-645): 1.97e-56: 202: 13.90		UBE2W_HUMAN (1-151): 1.05e-62: 190: 100.00
+	Hs
		849306 RNF4_HUMAN (160-344): 2.15e-37: 140: 20.00		UB2D1_HUMAN (4-150): 2.02e-69: 207: 34.69
+	Hs
		919705 RNF4_HUMAN (160-344): 2.15e-37: 140: 20.00		UB2D2_HUMAN (4-150): 1.14e-68: 205: 33.33
+	Hs
		895776 RNF4_HUMAN (160-344): 2.15e-37: 140: 20.00		UB2D3_HUMAN (4-150): 3.12e-67: 202: 33.33
+	Hs
		941269 RNF4_HUMAN (160-344): 2.15e-37: 140: 20.00		UB2E3_HUMAN (1-150): 1.13e-65: 200: 35.33
+	Hs
		851801 RNF4_HUMAN (160-344): 2.15e-37: 140: 20.00		UB2E1_HUMAN (1-150): 3.64e-64: 196: 34.00
+	Hs
		828982 RNF4_HUMAN (160-344): 2.15e-37: 140: 20.00		UBE2A_HUMAN (1-146): 7.59e-64: 193: 31.97
+	Hs
		927745 RNF4_HUMAN (160-344): 2.15e-37: 140: 20.00		UBE2B_HUMAN (1-146): 2.42e-63: 192: 33.33
+	Hs
		941279 RNF4_HUMAN (160-344): 2.15e-37: 140: 20.00		UBE2W_HUMAN (1-151): 1.05e-62: 190: 100.00