HitPredict

Interactor 1: SIAH1 (Q8IUQ4)
E3 ubiquitin-protein ligase SIAH1 (EC 2.3.2.27) (RING-type E3 ubiquitin transferase SIAH1) (Seven in absentia homolog 1) (Siah-1) (Siah-1a)
Gene: SIAH1

Interactor 2: AFF4 (Q9UHB7)
AF4/FMR2 family member 4 (ALL1-fused gene from chromosome 5q31 protein) (Protein AF-5q31) (Major CDK9 elongation factor-associated protein)
Gene: AFF4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.66	0.50	0.578

Property	Details
Experiments	25416956, 16189514
Identification Methods	two hybrid, two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	-
Common GO terms	-
Homologous interactions	10

Homologous interactions

Color coding for hit scores

	1 282 Hs			1 1163
+	Mm
		896601 SIA1A_MOUSE (1-282): 2.65e-120: 347: 99.65		AFF4_MOUSE (1-1163): 1.58e-120: 402: 91.25
+	Hs
		850816 SIAH1_HUMAN (1-282): 7.29e-121: 348: 100.00		AFF1_HUMAN (4-1160): 4.79e-88: 312: 34.47
+	Hs
		199100 SIAH2_HUMAN (28-282): 1.74e-103: 306: 83.20		AFF1_HUMAN (4-1160): 4.79e-88: 312: 34.47
+	Mm
		223089 SIA1A_MOUSE (1-282): 2.65e-120: 347: 99.65		AFF2_MOUSE (3-1162): 1.23e-73: 270: 29.51
+	Hs
		1216668 SIAH1_HUMAN (1-282): 7.29e-121: 348: 100.00		Q8TAX5_HUMAN (1-363): 3.06e-38: 149: 99.73
+	Hs
		1101024 RN151_HUMAN (35-150): 2.56e-11: 64: 26.02		AFF4_HUMAN (1-1163): 1.3e-124: 413: 100.00
+	Rn
		1265322 SIAH1_RAT (1-282): 2.65e-120: 347: 99.65		PCLO_RAT (24-928): 6.07e-19: 95.9: 11.72
+	Hs
		990867 TRI68_HUMAN (26-238): 2.54e-37: 139: 18.52		ZO1_HUMAN (80-902): 3.89e-34: 145: 12.83
+	Dm
		1370388 Q9W4W4_DROME (9-279): 4.15e-49: 173: 23.68		Q9VLS7_DROME (52-1070): 6.32e-13: 75.9: 7.69
+	Hs
		311832 RNF41_HUMAN (25-152): 1.42e-32: 123: 17.65		TACC2_HUMAN (32-1090): 5.83e-15: 82.4: 8.66