HitPredict

Interactor 1: RNF25 (Q96BH1)
E3 ubiquitin-protein ligase RNF25 (EC 2.3.2.27) (RING finger protein 25) (RING finger protein AO7)
Gene: RNF25

Interactor 2: UB2D4 (Q9Y2X8)
Ubiquitin-conjugating enzyme E2 D4 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme D4) (HBUCE1) (Ubiquitin carrier protein D4) (Ubiquitin-protein ligase D4)
Gene: UBE2D4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.87	1.00	0.935

Property	Details
Experiments	28514442, 36949045, 33961781, 19690564, 19549727
Identification Methods	affinity chromatography technology, two hybrid, two hybrid array, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	RWD~UQ_con, UQ_con~zf-RING_2
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0005634 - nucleus GO:0006511 - ubiquitin-dependent protein catabolic process GO:0016567 - protein ubiquitination GO:0085020 - protein K6-linked ubiquitination
Homologous interactions	14

Homologous interactions

Color coding for hit scores

	1 459 Hs			1 147
+	Mm
		919926 RNF25_MOUSE (1-459): 0: 669: 82.35		UB2D2_MOUSE (1-147): 1.54e-82: 240: 93.20
+	Hs
		849398 RNF25_HUMAN (1-459): 0: 651: 100.00		UB2D1_HUMAN (1-147): 4.61e-83: 241: 91.84
+	Mm
		854556 RNF25_MOUSE (1-459): 0: 669: 82.35		UB2E3_MOUSE (4-146): 1.24e-74: 223: 64.34
+	Hs
		919815 RNF25_HUMAN (1-459): 0: 651: 100.00		UB2D2_HUMAN (1-147): 1.54e-82: 240: 93.20
+	Hs
		895869 RNF25_HUMAN (1-459): 0: 651: 100.00		UB2D3_HUMAN (1-147): 1.56e-81: 238: 91.84
+	Mm
		854550 RNF25_MOUSE (1-459): 0: 669: 82.35		UB2E1_MOUSE (4-146): 2.56e-73: 219: 64.34
+	Hs
		1285490 RNF25_HUMAN (1-459): 0: 651: 100.00		UB2E2_HUMAN (4-146): 3.5e-75: 224: 64.34
+	Hs
		1282323 RNF25_HUMAN (1-459): 0: 651: 100.00		UB2E3_HUMAN (4-146): 1.24e-74: 223: 64.34
+	Hs
		851863 RNF25_HUMAN (1-459): 0: 651: 100.00		UB2E1_HUMAN (4-146): 4.67e-73: 218: 64.34
+	Dm
		54265 A1Z9I6_DROME (15-257): 1.93e-84: 265: 35.92		UBCD1_DROME (1-147): 4.66e-82: 239: 92.52
+	Dm
		54267 A1Z9I6_DROME (15-257): 1.93e-84: 265: 35.92		UBCD2_DROME (4-146): 7.36e-75: 224: 65.73
+	At
		1118123 Q4TU20_ARATH (23-277): 3.55e-80: 254: 25.09		UBC29_ARATH (1-147): 3.11e-79: 232: 76.87
+	At
		1258961 Q8W4A3_ARATH (128-277): 2.36e-44: 157: 24.16		UBC29_ARATH (1-147): 3.11e-79: 232: 76.87
+	Zm
		41536 A0A1D6NML1_MAIZE (33-127): 4.84e-25: 107: 25.00		B4FC97_MAIZE (1-147): 1.78e-83: 243: 79.59